Gene Expression Data
Assay Details
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Assay
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Reference:
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J:213418
Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7615138
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Probe:
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Gli1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2S:
embryonic day 10.5-10.75
(more  )
Note: 36-39-somite stage
Specimen
2S:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5-10.75
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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36-39-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
2S
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Expression marked the posterior region.
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Specimen
2T:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Irx3tm3.1Hui/Irx3tm3.1Hui
Irx5tm3Hui/Irx5tm3Hui
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Age:
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embryonic day 10.5-10.75
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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36-39-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
2T
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Expression domains were expanded to the anterior limb margin in the mutant.
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Specimen
3Q:
embryonic day 10.5
(more )
Specimen
3Q:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3Q
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Expressed in the posterior region.
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Specimen
3R:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3R
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Expression was slightly expanded anteriorly, compared with the control.
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Specimen
3S:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Kif7tm1.2Hui/Kif7tm1.2Hui
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3S
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Expression was expanded anteriorly, compared with the control.
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Specimen
3T:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
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3T
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Expression was expanded anteriorly, compared with the control.
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Specimen
4E:
embryonic day 10.0
(more )
Note: 30-somite stage
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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30-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
4E
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
4F:
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
4F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
4F
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
4G:
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
4G
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
4H:
embryonic day 10.5
(more )
Note: 38-somite stage
Specimen
4H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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38-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
4H
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
4K:
embryonic day 10.5
(more )
Specimen
4K:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
4K
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Expressed in the posterior region.
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Specimen
4L:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
4L
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Expression was expanded anteriorly, compared with the control.
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Specimen
4M:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
4M
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The expanded expression domain was reduced compared with the DKO hindlimb. A Gli1-negative region appeared in the anterior region.
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Specimen
S4L/L':
embryonic day 9.5
(more )
Note: 28-somite stage
Specimen
S4L/L':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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28-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
S4L
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S4L'
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4M/M':
embryonic day 9.5
(more )
Note: 29-somite stage
Specimen
S4M/M':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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29-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
S4M
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S4M'
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4N/N':
embryonic day 10.0
(more )
Note: 30-somite stage
Specimen
S4N/N':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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30-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4N
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S4N'
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4O/O':
embryonic day 10.0
(more )
Note: 32-somite stage
Specimen
S4O/O':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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32-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4O'
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S4O
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4P/P':
embryonic day 10.0
(more )
Note: 33-somite stage
Specimen
S4P/P':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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33-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4P
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S4P'
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4Q/Q':
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
S4Q/Q':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4Q'
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S4Q
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4R/R':
embryonic day 10.0
(more )
Note: 33-somite stage
Specimen
S4R/R':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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33-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
S4R
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S4R'
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4S/S':
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
S4S/S':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
S4S
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S4S'
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4T/T':
embryonic day 10.5
(more )
Note: 35-somite stage
Specimen
S4T/T':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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35-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4T'
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S4T
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4U/U':
embryonic day 10.5
(more )
Note: 37-somite stage
Specimen
S4U/U':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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37-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4U'
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S4U
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4V/V':
embryonic day 10.5
(more )
Note: 38-somite stage
Specimen
S4V/V':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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38-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4V
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S4V'
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Specimen
S4W/W':
embryonic day 10.5
(more )
Note: 39-somite stage
Specimen
S4W/W':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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39-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4W'
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S4W
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Expressed more posteriorly restricted (a broader Gli1-negative anterior domain) in the forelimb relative to the hindlimb.
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Analysis Tools