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Gene Expression Data
Assay Details
Assay
Reference: J:293098 Jafree DJ, et al., Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease. Elife. 2019 Dec 6;8:e48183
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7614746
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: anti-Prox1 (AF2727)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Assay notes: Depending on the different antibody combination in the double/triple labeling, another antibody (MGI:7328614) was used.
Results
Specimen 1A E14.5: embryonic day 14.5 (more )
Note: Double labeled: purple - Prox1; green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS22: renal lymphatic endothelial cell of lymphatic vessel Present 1A E14.5 PROX1 , 1A E14.5 merge lower , 1A E14.5 merge upper A cellular network of PROX1+/LYVE-1+ lymphatic endothelial cells was first seen.

Specimen 1A E16.5: embryonic day 16.5 (more )
Note: Double labeled: purple - Prox1; green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 1A E16.5 PROX1 , 1A E16.5 merge lower , 1A E16.5 merge upper The positive kidney lymphatics constituted a vascular plexus, that contained some lumenized vessel segments.
TS24: renal tubule kidney tubule cell Weak 1A E16.5 PROX1 , 1A E16.5 merge lower

Specimen 1A E18.5: embryonic day 18.5 (more )
Note: Double labeled: purple - Prox1; green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS26: renal lymphatic endothelial cell of lymphatic vessel Present 1A E18.5 PROX1 , 1A E18.5 merge upper , 1A E18.5 merge lower Lymphatic vessel branches were observed distally in the kidney.
TS26: renal tubule kidney tubule cell Weak 1A E18.5 merge lower , 1A E18.5 PROX1

Specimen 1-S1 E15.5 PROX1/VEGFR-3: embryonic day 15.5 (more )
Note: Double labeled: purple - Prox1; green - Flt4 (assay MGI:7614752).
Structure Cell Type Level Pattern Image Note
TS23: renal lymphatic endothelial cell of lymphatic vessel Present 1-S1_merge upper right , 1-S1_upper PROX1 , 1-S1_merge upper left Coexpressed with Flt4/VEGFR3 in the lymphatic plexus.

Specimen 1-S1 E16.5 PROX1/Podoplanin: embryonic day 16.5 (more )
Note: Double labeled: purple - Prox1; green - Pdpn (assay MGI:7614751).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 1-S1_middle PROX1 , 1-S1_merge middle right , 1-S1_merge middle left Coexpressed with Emcn in the lymphatic plexus.

Specimen 1-S1 E15.5 PROX1/LYVE-1/Endomucin: embryonic day 15.5 (more )
Note: Triple labeled: purple - Prox1; red - Emcn (assay MGI:7614754); green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS23: renal lymphatic endothelial cell of lymphatic vessel Present 1-S1_merge lower right , 1-S1_lower PROX1 , 1-S1_merge lower left Coexpressed with Lyve1 in the lymphatic plexus. Weak expression of the blood endothelial marker endomucin relative to surrounding blood vasculature.

Specimen 1-S2A: embryonic day 16.5 (more )
Note: Triple labeled: purple - Prox1; green - Pdpn (assay MGI:7614751); blue - Upk3a (assay MGI:7614779).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 1-S2A PROX1 , 1-S2A merge right , 1-S2A merge left The ring-like lymphatic anastomosis resided under the nascent Upk3a+ renal pelvis.

Specimen 1-S2B: embryonic day 17.5 (more )
Note: Triple labeled: purple - Prox1; green - Lyve1 (assay MGI:7614745); red - Pecam1 (assay MGI:7614827).
Structure Cell Type Level Pattern Image Note
TS25: renal lymphatic endothelial cell of lymphatic vessel Present 1-S2B PROX1 , 1-S2B merge right , 1-S2B merge left PECAM1+ blood endothelium and lymphatic vasculature run alongside each other.

Specimen 1-S2C: embryonic day 18.5 (more )
Note: Triple labeled: purple - Prox1; blue - Acta2 (assay MGI:7614777); green - Pecam1 (assay MGI:7614838).
Structure Cell Type Level Pattern Image Note
TS26: renal lymphatic endothelial cell of lymphatic vessel Present 1-S2C merge left , 1-S2C PROX1 , 1-S2C merge right PECAM1+ blood endothelium and lymphatic vasculature run alongside each other. No Acta2/alphaSMA+ cells around PROX1+ vessels.

Specimen 1-S2D: embryonic day 18.5 (more )
Note: Triple labeled: purple - Prox1; brown - Aqp2 (assay MGI:7614774); green - Pdpn (assay MGI:7614751).
Structure Cell Type Level Pattern Image Note
TS26: renal lymphatic endothelial cell of lymphatic vessel Present 1-S2D PROX1 , 1-S2D merge right , 1-S2D merge left Positive lymphatic vessels were observed in the hilum. No lymphatic vessels in the renal papilla or medulla region (Aqp2+), nor near or within the renal capsule.

Specimen 2A: embryonic day 16.5 (more )
Note: Double labeled: purple - Prox1; green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 2A merge , 2A PROX1 PROX1+/LYVE1+ lymphatic endothelial cells appeared as either vessels or cell clusters.

Specimen 2B PROX1/LYVE-1: embryonic day 16.5 (more )
Note: Double labeled: purple - Prox1; green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 2B PROX1 left , 2B merge left Coexpressed with Lyve1 in cell clusters.

Specimen 2B PROX1/Podoplanin: embryonic day 16.5 (more )
Note: Double labeled: purple - Prox1; green - Pdpn (assay MGI:7614751).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 2B merge middle , 2B PROX1 middle Coexpressed with Pdpn in cell clusters.

Specimen 2B PROX1/VEGFR-3: embryonic day 15.5 (more )
Note: Double labeled: purple - Prox1; green - Flt4 (assay MGI:7614752).
Structure Cell Type Level Pattern Image Note
TS23: renal lymphatic endothelial cell of lymphatic vessel Present 2B PROX1 right , 2B merge right Coexpressed with Flt4/Vegfr3 in cell clusters.

Specimen 2-S1 PROX1/LYVE-1/PECAM-1: embryonic day 15.5 (more )
Note: Triple labeled: purple - Prox1; green - Lyve1 (assay MGI:7614745); red - Pecam1 (assay MGI:7614827).
Structure Cell Type Level Pattern Image Note
TS23: renal lymphatic endothelial cell of lymphatic vessel Present 2-S1_merge left , 2-S1_PROX1 left Coexpressed with Lyve1 in cell clusters.

Specimen 2-S1 PROX1/LYVE-1/Endomucin: embryonic day 15.5 (more )
Note: Triple labeled: purple - Prox1; blue - Emcn (assay MGI:7614847); green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS23: renal lymphatic endothelial cell of lymphatic vessel Present 2-S1_merge middle , 2-S1_PROX1 middle Coexpressed with Lyve1 in cell clusters.

Specimen 2-S1 PROX1/LYVE-1/F4/80: embryonic day 15.5 (more )
Note: Triple labeled: purple - Prox1; brown - Adgre1 (assay MGI:7614850); green - Lyve1 (assay MGI:7614745).
Structure Cell Type Level Pattern Image Note
TS23: renal lymphatic endothelial cell of lymphatic vessel Present 2-S1_PROX1 right , 2-S1_merge right Coexpressed with Lyve1 in cell clusters.


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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory