Antibody:	anti-LYVE1 (AF2125)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Depending on the different antibody combination in the double/triple labeling, another antibody (MGI:6314682) was used.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: renal lymphatic	endothelial cell of lymphatic vessel	Present		1A E14.5 LYVE-1 , 1A E14.5 merge upper , 1A E14.5 merge lower	A cellular network of PROX1+/LYVE-1+ lymphatic endothelial cells was first seen.
TS22: renal interstitium	kidney resident macrophage	Strong		1A E14.5 merge lower , 1A E14.5 merge upper , 1A E14.5 LYVE-1

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		1A E16.5 LYVE-1 , 1A E16.5 merge upper , 1A E16.5 merge lower	The positive kidney lymphatics constituted a vascular plexus, that contained some lumenized vessel segments.
TS24: renal interstitium	kidney resident macrophage	Strong		1A E16.5 merge upper , 1A E16.5 LYVE-1 , 1A E16.5 merge lower

Structure	Cell Type	Level	Pattern	Image	Note
TS26: renal lymphatic	endothelial cell of lymphatic vessel	Present		1A E18.5 merge upper , 1A E18.5 merge lower , 1A E18.5 LYVE-1	Positive lymphatic vessel branches were observed distally in the kidney.
TS26: renal interstitium	kidney resident macrophage	Strong		1A E18.5 merge upper , 1A E18.5 merge lower , 1A E18.5 LYVE-1

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S1_merge lower left , 1-S1_merge lower right	Coexpressed with Prox1 in the lymphatic plexus. Weak expression of the blood endothelial marker endomucin relative to surrounding blood vasculature.
TS23: renal interstitium	kidney resident macrophage	Strong		1-S1_merge lower left , 1-S1_merge lower right

Structure	Cell Type	Level	Pattern	Image	Note
TS25: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S2B merge left , 1-S2B LYVE-1 , 1-S2B merge right	Positive lymphatic vasculature run alongside with the PECAM1+ blood endothelium.
TS25: renal interstitium	kidney resident macrophage	Strong		1-S2B merge left , 1-S2B merge right , 1-S2B LYVE-1

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		2A merge , 2A LYVE-1	PROX1+/LYVE1+ lymphatic endothelial cells appeared as either vessels or cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		2B LYVE-1 , 2B merge left	Coexpressed with Prox1 in cell clusters.
TS24: renal interstitium	kidney resident macrophage	Strong		2B merge left , 2B LYVE-1

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		2-S1_LYVE-1 left , 2-S1_merge left	Coexpressed with Prox1 in cell clusters.
TS23: renal interstitium	kidney resident macrophage	Present		2-S1_LYVE-1 left , 2-S1_merge left

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		2-S1_merge middle , 2-S1_LYVE-1 middle	Coexpressed with Prox1 in cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		2-S1_merge right , 2-S1_LYVE-1 right	Coexpressed with Prox1 in cell clusters.
TS23: renal interstitium	kidney resident macrophage	Present		2-S1_merge right , 2-S1_LYVE-1 right