Antibody:	anti-Prox1 (AF2727)
Detection system:	Secondary antibody coupled to Alexa Fluor 546
Assay notes:	Depending on the different antibody combination in the double/triple labeling, another antibody (MGI:7328614) was used.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: renal lymphatic	endothelial cell of lymphatic vessel	Present		1A E14.5 PROX1 , 1A E14.5 merge lower , 1A E14.5 merge upper	A cellular network of PROX1+/LYVE-1+ lymphatic endothelial cells was first seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		1A E16.5 PROX1 , 1A E16.5 merge lower , 1A E16.5 merge upper	The positive kidney lymphatics constituted a vascular plexus, that contained some lumenized vessel segments.
TS24: renal tubule	kidney tubule cell	Weak		1A E16.5 PROX1 , 1A E16.5 merge lower

Structure	Cell Type	Level	Pattern	Image	Note
TS26: renal lymphatic	endothelial cell of lymphatic vessel	Present		1A E18.5 PROX1 , 1A E18.5 merge upper , 1A E18.5 merge lower	Lymphatic vessel branches were observed distally in the kidney.
TS26: renal tubule	kidney tubule cell	Weak		1A E18.5 merge lower , 1A E18.5 PROX1

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S1_merge upper right , 1-S1_upper PROX1 , 1-S1_merge upper left	Coexpressed with Flt4/VEGFR3 in the lymphatic plexus.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S1_middle PROX1 , 1-S1_merge middle right , 1-S1_merge middle left	Coexpressed with Emcn in the lymphatic plexus.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S1_merge lower right , 1-S1_lower PROX1 , 1-S1_merge lower left	Coexpressed with Lyve1 in the lymphatic plexus. Weak expression of the blood endothelial marker endomucin relative to surrounding blood vasculature.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S2A PROX1 , 1-S2A merge right , 1-S2A merge left	The ring-like lymphatic anastomosis resided under the nascent Upk3a+ renal pelvis.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S2B PROX1 , 1-S2B merge right , 1-S2B merge left	PECAM1+ blood endothelium and lymphatic vasculature run alongside each other.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S2C merge left , 1-S2C PROX1 , 1-S2C merge right	PECAM1+ blood endothelium and lymphatic vasculature run alongside each other. No Acta2/alphaSMA+ cells around PROX1+ vessels.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: renal lymphatic	endothelial cell of lymphatic vessel	Present		1-S2D PROX1 , 1-S2D merge right , 1-S2D merge left	Positive lymphatic vessels were observed in the hilum. No lymphatic vessels in the renal papilla or medulla region (Aqp2+), nor near or within the renal capsule.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		2A merge , 2A PROX1	PROX1+/LYVE1+ lymphatic endothelial cells appeared as either vessels or cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		2B PROX1 left , 2B merge left	Coexpressed with Lyve1 in cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: renal lymphatic	endothelial cell of lymphatic vessel	Present		2B merge middle , 2B PROX1 middle	Coexpressed with Pdpn in cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		2B PROX1 right , 2B merge right	Coexpressed with Flt4/Vegfr3 in cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		2-S1_merge left , 2-S1_PROX1 left	Coexpressed with Lyve1 in cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		2-S1_merge middle , 2-S1_PROX1 middle	Coexpressed with Lyve1 in cell clusters.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: renal lymphatic	endothelial cell of lymphatic vessel	Present		2-S1_PROX1 right , 2-S1_merge right	Coexpressed with Lyve1 in cell clusters.