Gene Expression Data
Assay Details
Assay
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Reference:
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J:173957
Tsutsumi M, et al., Characterization of a Novel Mouse Gene Encoding an SYCP3-Like Protein That Relocalizes from the XY Body to the Nucleolus During Prophase of Male Meiosis I. Biol Reprod. 2011 Jul;85(1):165-71
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7610715
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Gene symbol:
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1700013H16Rik
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Gene name:
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RIKEN cDNA 1700013H16 gene
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Antibody:
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anti-XLR6
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A Spermatocytes Leptotene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Spermatocytes Leptotene:
(close )
Genetic Background:
|
DDY
|
Age:
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postnatal adult
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
Diffuse |
3A sperm Lep XLR6
,
3A sperm Lep merge
|
Diffusely, but not uniformly, distributed throughout the nuclei.
|
Specimen
3A Spermatocytes Zygotene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Spermatocytes Zygotene:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
Diffuse |
3A sperm Zyg merge
,
3A sperm Zyg XLR6
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Diffusely, but not uniformly, distributed throughout the nuclei.
|
Specimen
3A Spermatocytes Pachytene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Triple labeled: green - 1700013H16Rik; blue - H2ax (assay MGI:7610717); red - Sycp3 (assay MGI:7610716).
Specimen
3A Spermatocytes Pachytene:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Triple labeled: green - 1700013H16Rik; blue - H2ax (assay MGI:7610717); red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Strong |
Regionally restricted |
3A sperm Pach merge
,
3A sperm Pach XLR6
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Expressed in the the XY body (coexpressed with H2ax).
|
Specimen
3A Spermatocytes Diplotene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Spermatocytes Diplotene:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Strong |
Regionally restricted |
3A sperm Dip XLR6
,
3A sperm Dip merge
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Expressed in the the XY body.
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Specimen
3A Spermatocytes Diakinesis:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Spermatocytes Diakinesis:
(close )
Genetic Background:
|
DDY
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Age:
|
postnatal adult
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Sex:
|
Male
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Absent |
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3A sperm Dia XLR6
,
3A sperm Dia merge
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Absent in diakinesis stage, accordance with the disappearance of the XY body.
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Specimen
3A Oocyte Leptotene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Oocyte Leptotene:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Diffuse |
3A oocyte Lep XLR6
,
3A oocyte Lep merge
|
Diffusely, but not uniformly, distributed throughout the nuclei.
|
Specimen
3A Oocyte Zygotene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Oocyte Zygotene:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Diffuse |
3A oocyte Zyg merge
,
3A oocyte Zyg XLR6
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Diffusely, but not uniformly, distributed throughout the nuclei.
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Specimen
3A Oocyte Pachytene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Oocyte Pachytene:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Absent |
|
3A oocyte Pach merge
,
3A oocyte Pach XLR6
|
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Specimen
3A Oocyte Diplotene:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3A Oocyte Diplotene:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Absent |
|
3A oocyte Dip XLR6
,
3A oocyte Dip merge
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Specimen
3B Pachytene oocyte:
postnatal adult
(more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Specimen
3B Pachytene oocyte:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
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Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
3B XLR6
,
3B merge
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Signals were observed at the unpaired chromosomes occasionally found in the pachytene oocytes with pairing failure.
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Specimen
4A Early-pachy:
postnatal adult
(more )
Note: Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
Specimen
4A Early-pachy:
(close )
Genetic Background:
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DDY
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
Regionally restricted |
4A early merge
,
4A early XLR6
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Expressed in the the XY body (coexpressed with H2ax).
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TS28: round spermatid |
Absent |
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4A early merge
,
4A early XLR6
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Specimen
4A Mid-pachy:
postnatal adult
(more )
Note: Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
Specimen
4A Mid-pachy:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal adult
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Sex:
|
Male
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
Regionally restricted |
4A mid XLR6
,
4A mid merge
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Expressed in the the XY body (coexpressed with H2ax).
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TS28: round spermatid |
Absent |
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4A mid merge
,
4A mid XLR6
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TS28: spermatogonium |
Present |
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4A mid merge
,
4A mid XLR6
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Expressed in the nuclei of type B spermatogonia.
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Specimen
4A Late-pachy:
postnatal adult
(more )
Note: Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
Specimen
4A Late-pachy:
(close )
Genetic Background:
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DDY
|
Age:
|
postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
Regionally restricted |
4A late XLR6
,
4A late merge
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Concentrated in a distinct nuclear compartment at the center side of the H2ax-stained XY body but could still be observed at the nuclear periphery.
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Specimen
4B Late-diplo:
postnatal adult
(more )
Note: Double labeled: green - 1700013H16Rik; red - H2ax (assay MGI:7610718).
Specimen
4B Late-diplo:
(close )
Genetic Background:
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DDY
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Age:
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postnatal adult
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Sex:
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Male
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - 1700013H16Rik; red - H2ax (assay MGI:7610718).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Present |
|
4B merge
,
4B XLR6
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Signals were separate from those of the XY body and appeared at the center of the nuclei.
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