H2ax Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:173957 Tsutsumi M, et al., Characterization of a Novel Mouse Gene Encoding an SYCP3-Like Protein That Relocalizes from the XY Body to the Nucleolus During Prophase of Male Meiosis I. Biol Reprod. 2011 Jul;85(1):165-71
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7610717
Gene symbol:	H2ax
Gene name:	H2A.X variant histone

Antibody:	anti-gammaH2AX
Detection system:	Secondary antibody coupled to other - see notes

Results

Specimen 3A Spermatocytes Pachytene: postnatal adult (more

)
Note: Meiotic nuclear spreads, with aminomethylcoumarin acetate-conjugated secondary antibody. Triple labeled: blue - H2ax; green - 1700013H16Rik (assay MGI:7610715); red - Sycp3 (assay MGI:7610716).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	3A sperm Pach merge	Expressed in the the XY body.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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