Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:203122
Cioffi S, et al., Tbx1 regulates brain vascularization. Hum Mol Genet. 2014 Jan 1;23(1):78-89
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7610005
|
|
Gene symbol:
|
Slc2a1
|
|
Gene name:
|
solute carrier family 2 (facilitated glucose transporter), member 1
|
|
Antibody:
|
anti-Glut1
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
|
Results
|
Specimen
2A:
embryonic day 18.5
(more  )
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
2A':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm1Bld/Tbx1tm1Bld
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
2B:
embryonic day 18.5
(more )
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
2B':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm1Bld/Tbx1tm1Bld
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain blood vessel |
Present |
Regionally restricted |
2B'
|
Number of vessel branch points increased 37.2% compared to wild type.
|
Specimen
2C:
embryonic day 18.5
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
2C':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm1Bld/Tbx1tm1Bld
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain blood vessel |
Present |
Regionally restricted |
2C'
|
Increased number of filopodial bursts compared to control.
|
Specimen
2D:
embryonic day 18.5
(more )
Specimen
2D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
2D':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm1Bld/Tbx1tm1Bld
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain blood vessel |
Present |
Regionally restricted |
2D'
|
Disorganization of brain vascular network characterized by increased number of microcirculatory loops.
|
Specimen
S2A,a:
embryonic day 13.5
(more )
Specimen
S2A,a:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
S2A',a':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm1Bld/Tbx1tm1Bld
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brain blood vessel |
Present |
Regionally restricted |
S2A'
,
S2a'
|
Authors report brain vessel hyperplasia.
|
Specimen
S2B,b:
embryonic day 15.5
(more )
Specimen
S2B,b:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
S2B',b':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm1Bld/Tbx1tm1Bld
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: brain blood vessel |
Present |
Regionally restricted |
S2B'
,
S2b'
|
Authors report brain vessel hyperplasia.
|
Specimen
3A,B:
postnatal month 8
(more )
Specimen
3A,B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal month 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
3A',B':
postnatal month 8; Tbx1tm1Bld/Tbx1 +
(more )
Specimen
3A',B':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm1Bld/Tbx1+
|
|
Age:
|
postnatal month 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain blood vessel |
Present |
Regionally restricted |
3B'
,
3A'
|
Authors report mild brain vessel hyperplasia and disorganization.
|
Specimen
5A:
embryonic day 18.5; Tbx1tm3Bld/Tbx1 +
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm3Bld/Tbx1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
5A':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6NCrlj * CBA/JNCrlj
|
|
Mutant Allele(s):
|
Mesp1tm2(cre)Ysa/Mesp1+
Tbx1tm3Bld/Tbx1tm3Bld
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Conditional mutant.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain blood vessel |
Present |
Regionally restricted |
5A'
|
Brain vessel density increased 32.7% compared to controls
|
Specimen
5B:
embryonic day 18.5; Tbx1tm3Bld/Tbx1 +
(more )
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm3Bld/Tbx1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
5B':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Tbx1tm3Bld/Tbx1tm3Bld
Tg(Tek-cre)1Ywa/0
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Conditional mutant.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain blood vessel |
Present |
Regionally restricted |
5B'
|
Brain vessel density increased 32% compared to controls.
|
Specimen
5C:
embryonic day 18.5; Tbx1tm3Bld/Tbx1 +
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm3Bld/Tbx1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
5C':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Tbx1tm3Bld/Tbx1tm3Bld
Tg(Tek-cre)1Ywa/0
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Conditional mutant.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain blood vessel |
Present |
Regionally restricted |
5C'
|
Increased number of vessel branch points compared to control.
|
Specimen
5D:
embryonic day 18.5; Tbx1tm3Bld/Tbx1 +
(more )
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Tbx1tm3Bld/Tbx1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
Specimen
5D':
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Tbx1tm3Bld/Tbx1tm3Bld
Tg(Tek-cre)1Ywa/0
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Agarose
|
|
Note:
|
Conditional mutant.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain blood vessel |
Present |
Regionally restricted |
5D'
|
Increased number of endothelial sprouts compared to control.
|
|
Analysis Tools