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Gene Expression Data
Assay Details
Assay
Reference: J:262420 Szenker-Ravi E, et al., RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6. Nature. 2018 May;557(7706):564-569
Assay type: RNA in situ
MGI Accession ID: MGI:7596266
Gene symbol: Rspo2
Gene name: R-spondin 2
Probe: Mm-Rspo2 (402001)
Probe preparation: Antisense DNA
Visualized with: Chromogenic
Results
Specimen 2h Lgr4/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr4 (assay MGI:7596252).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Lgr4/Rspo2 (upper) Strong expression co-localized with Lgr4 in apical ectodermal ridge.
Specimen 2h Lgr4/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr4 (assay MGI:7596252).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Lgr4/Rspo2 (lower) Strong expression co-localized with Lgr4 in apical ectodermal ridge.
Specimen 2h Lgr5/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr5 (assay MGI:7596253).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Lgr5/Rspo2 (upper) Strong expression co-localized with Lgr5 in apical ectodermal ridge.
Specimen 2h Lgr5/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr5 (assay MGI:7596253).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Lgr5/Rspo2 (lower) Strong expression co-localized with Lgr5 in apical ectodermal ridge.
Specimen 2h Lgr6/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr6 (assay MGI:7596255).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Lgr6/Rspo2 (upper) Strong expression in apical ectodermal ridge.
Specimen 2h Lgr6/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr6 (assay MGI:7596255).
Structure Level Pattern Image Note
TS22: forelimb epidermis Present Regionally restricted 2h Lgr6/Rspo2 (lower) Strong expression in apical ectodermal ridge.
Specimen 2h Rnf43/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Rnf43 (assay MGI:7596256).
Structure Level Pattern Image Note
TS22: forelimb epidermis Present Regionally restricted 2h Rnf43/Rspo2 (upper) Strong expression in apical ectodermal ridge.
Specimen 2h Rnf43/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Rnf43 (assay MGI:7596256).
Structure Level Pattern Image Note
TS22: forelimb epidermis Present Regionally restricted 2h Rnf43/Rspo2 (lower) Strong expression in apical ectodermal ridge.
Specimen 2h Znrf3/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Znrf3 (assay MGI:7596259).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Znrf3/Rspo2 (upper) Strong expression in apical ectodermal ridge.
Specimen 2h Znrf3/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Znrf3 (assay MGI:7596259).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Znrf3/Rspo2 Ilower) Strong expression in apical ectodermal ridge.
Specimen 2h Wnt3/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Wnt3 (assay MGI:7596263).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Wnt3/Rspo2 (upper) Strong expression in apical ectodermal ridge.
Specimen 2h Wnt3/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Wnt3 (assay MGI:7596263).
Structure Level Pattern Image Note
TS22: forelimb epidermis Strong Regionally restricted 2h Wnt3/Rspo2 (lower) Strong expression in apical ectodermal ridge.
Specimen ED5f Lgr4/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr4 (assay MGI:7596252).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Lgr4/Rspo2 (upper)
Specimen ED5f Lgr4/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr4 (assay MGI:7596252).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Lgr4/Rspo2 (lower)
Specimen ED5f Lgr5/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr5 (assay MGI:7596253).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Lgr5/Rspo2 (upper)
Specimen ED5f Lgr5/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr5 (assay MGI:7596253).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Lgr5/Rspo2 (lower)
Specimen ED5f Lgr6/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr6 (assay MGI:7596255).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Lgr6/Rspo2 (upper)
Specimen ED5f Lgr6/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Lgr6 (assay MGI:7596255).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Lgr6/Rspo2 (lower)
Specimen ED5f Rnf43/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Rnf43 (assay MGI:7596256).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Rnf43/Rspo2 (upper)
Specimen ED5f Rnf43/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Rnf43 (assay MGI:7596256).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Rnf43/Rspo2 (lower)
Specimen ED5f Znrf3/Rspo2 (upper): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Znrf3 (assay MGI:7596259).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Znrf3/Rspo2 (upper)
Specimen ED5f Znrf3/Rspo2 (lower): embryonic day 14.5 (more )
Note: Double labeled: pink - Rspo2; blue - Znrf3 (assay MGI:7596259).
Structure Level Pattern Image Note
TS22: lung mesenchyme Present ED5f Znrf3/Rspo2 (lower)
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory