Gene Expression Data
Assay Details
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Assay
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Reference:
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J:333825
Lorenzana-Carrillo MA, et al., TRIM35-mediated degradation of nuclear PKM2 destabilizes GATA4/6 and induces P53 in cardiomyocytes to promote heart failure. Sci Transl Med. 2022 Nov 2;14(669):eabm3565
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7581047
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Gene symbol:
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Pkm
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Gene name:
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pyruvate kinase, muscle
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Antibody:
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PKM2(phospho-Ser37) Antibody #11456
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The antibody used recognizes the phosphorylated form of the protein.
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Results
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Specimen
1E:
postnatal adult
(more  )
Note: Triple labeled: green - Pkm; magenta - Vim (assay MGI:7581048); red - MHC.
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: green - Pkm; magenta - Vim (assay MGI:7581048); red - MHC.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: myocardium layer |
cardiac muscle cell |
Present |
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1E PKM2
,
1E merge
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Expressed in the nuclei of cardiomyocytes stained with myosin heavy chain (MHC).
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| TS28: heart |
fibroblast of cardiac tissue |
Absent |
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1E merge
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1E PKM2
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No expression in nonmyocytes (mostly fibroblasts) stained with vimentin.
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Analysis Tools
