About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:333825 Lorenzana-Carrillo MA, et al., TRIM35-mediated degradation of nuclear PKM2 destabilizes GATA4/6 and induces P53 in cardiomyocytes to promote heart failure. Sci Transl Med. 2022 Nov 2;14(669):eabm3565
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7581046
Gene symbol: Pkm
Gene name: pyruvate kinase, muscle
Antibody: PKM2 (D78A4) Rabbit mAb (4053)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1D: postnatal adult (more )
Note: Triple labeled: green - Pkm; magenta - Vim (assay MGI:7581048); red - MHC.
Structure Cell Type Level Pattern Image Note
TS28: myocardium layer cardiac muscle cell Present 1D PKM2 , 1D merge Expressed in the nuclei of cardiomyocytes stained with myosin heavy chain (MHC).
TS28: heart fibroblast of cardiac tissue Present 1D merge , 1D PKM2 Expressed in the cytoplasm of nonmyocytes (mostly fibroblasts) stained with vimentin.
Specimen S5B: postnatal adult; Pkmtm1.1Mgvh/Pkmtm1.1Mgvh, Tg(Myh6-cre)2182Mds/0 (more )
Note: Conditional mutant. Triple labeled: green - Pkm; magenta - Vim (assay MGI:7581048); red - MHC.
Structure Cell Type Level Pattern Image Note
TS28: myocardium layer cardiac muscle cell Absent S5B PKM2 , S5B merge No expression in the cardiomyocytes stained with myosin heavy chain (MHC) in the mutant.
TS28: heart fibroblast of cardiac tissue Present S5B merge , S5B PKM2 Expression in the cytoplasm of nonmyocytes (mostly fibroblasts) stained with vimentin remained.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/30/2025
MGI 6.24 		The Jackson Laboratory