Gene Expression Data
Assay Details
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Assay
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Reference:
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J:93057
Schuurmans C, et al., Sequential phases of cortical specification involve Neurogenin-dependent and -independent pathways. EMBO J. 2004 Jul 21;23(14):2892-902
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7578972
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Gene symbol:
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Robo1
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Gene name:
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roundabout guidance receptor 1
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Probe:
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Robo1 cDNA
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Probe preparation:
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Antisense RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2E:
embryonic day 15.5
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neurog2tm2Fgu/Neurog2tm2Fgu
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical plate |
Present |
Regionally restricted |
2F
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A correctly specified upper layer above distinct gaps in lower layer expression was seen.
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Specimen
5E:
postnatal day 7
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neurog2tm2Fgu/Neurog2tm2Fgu
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cortical layer V |
Present |
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5F
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A thinner, disorganized layer was seen, compared with the wild-type.
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Analysis Tools
