Gene Expression Data
Assay Details
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Assay
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Reference:
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J:316482
Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7576023
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2B lateral to medial 1:
embryonic day 12.5
(more  )
Note: Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
Specimen
2B lateral to medial 1:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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2B-1
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
2B lateral to medial 2:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
Specimen
2B lateral to medial 2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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2B-2
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
2B lateral to medial 3:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
Specimen
2B lateral to medial 3:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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2B-3
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
2B lateral to medial 4:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
Specimen
2B lateral to medial 4:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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2B-4
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
2B lateral to medial 5:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
Specimen
2B lateral to medial 5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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2B-5
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
2B lateral to medial 6:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
Specimen
2B lateral to medial 6:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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2B-6
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
2B lateral to medial 7:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
Specimen
2B lateral to medial 7:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Nkx2-9 (assay MGI:7576004).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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2B-7
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
6B +/+ rostral to caudal 1:
postnatal month 2.5
(more )
Specimen
6B +/+ rostral to caudal 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B +/+ rostral to caudal 2:
postnatal month 2.5
(more )
Specimen
6B +/+ rostral to caudal 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B +/+ rostral to caudal 3:
postnatal month 2.5
(more )
Specimen
6B +/+ rostral to caudal 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B LacZ/LacZ rostral to caudal 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B LacZ/LacZ rostral to caudal 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B LacZ/LacZ rostral to caudal 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6E +/+:
postnatal month 2.5
(more )
Specimen
6E +/+:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6E LacZ/LacZ:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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postnatal month 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools