Gene Expression Data
Assay Details
Assay
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Reference:
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J:63172
Erskine L, et al., Retinal ganglion cell axon guidance in the mouse optic chiasm: expression and function of robos and slits. J Neurosci. 2000 Jul 1;20(13):4975-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7565484
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Gene symbol:
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Robo1
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Gene name:
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roundabout guidance receptor 1
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Probe:
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Robo1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2B:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
|
vibratome section
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Specimen
2G/P:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
2G/P:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: retina |
retinal ganglion cell |
Present |
Regionally restricted |
2P
,
2G
|
Expressed throughout retinal ganglion cell layer, although restricted to a scattered subpopulation of cells.
|
Specimen
2L:
embryonic day 17.5
(more )
Note: vibratome section
Specimen
2L:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: retina layer |
Present |
Regionally restricted |
2L
|
Expressed at the inner nuclear layer.
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Specimen
3E:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3E
|
Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.
|
Specimen
3F:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3F
|
Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.
|
Specimen
3G:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3G:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3G
|
Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.
|
Specimen
3H:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3H:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3H
|
Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.
|
Specimen
4E:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4E
|
More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.
|
Specimen
4F:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4F
|
More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.
|
Specimen
4G:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4G:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4G
|
More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.
|
Specimen
4H:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4H:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4H
|
More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.
|
Specimen
5B:
embryonic day 17.5
(more )
Note: vibratome section
Specimen
5B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: diencephalon |
Present |
Regionally restricted |
5B
|
More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.
|
Specimen
not shown:
embryonic day 15.5
(more )
Note: vibratome section
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: retina layer |
retinal ganglion cell |
Present |
Regionally restricted |
|
Expressed throughout retinal ganglion cell layer.
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TS23: diencephalon |
|
Present |
Regionally restricted |
|
More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.
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