Syngap1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:334714 Yang R, et al., Upregulation of SYNGAP1 expression in mice and human neurons by redirecting alternative splicing. Neuron. 2023 Mar 3;
Assay type:	RT-PCR
MGI Accession ID:	MGI:7564411
Gene symbol:	Syngap1
Gene name:	synaptic Ras GTPase activating protein 1 homolog (rat)

Probe:

CH743-CH744

Results

Image: 4C

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	P10	TS28: neocortex	Present (b)	Present	Not Specified	C57BL/6		Not Specified
+/+	P10	TS28: neocortex	Present (d)	Present	Not Specified	C57BL/6		Not Specified
N/+	P10	TS28: neocortex	Weak (e)	Present	Not Specified	C57BL/6-Syngap1^em1Xiaoz	Syngap1^em1Xiaoz/Syngap1⁺	Not Specified
N/+	P10	TS28: neocortex	Weak (f)	Present	Not Specified	C57BL/6-Syngap1^em1Xiaoz	Syngap1^em1Xiaoz/Syngap1⁺	Not Specified
N/N	P10	TS28: neocortex	Absent (g)	Present	Not Specified	C57BL/6-Syngap1^em1Xiaoz	Syngap1^em1Xiaoz/Syngap1^em1Xiaoz	Not Specified
N/N	P10	TS28: neocortex	Absent (g)	Present	Not Specified	C57BL/6-Syngap1^em1Xiaoz	Syngap1^em1Xiaoz/Syngap1^em1Xiaoz	Not Specified

Notes:
(a) Larger band, alternative 3' splice site (A3SS)
(b) PSI (percent splice insertion) = 17% A3SS/(total transcripts)
(c) Smaller band.
(d) PSI = 12% A3SS/(total transcripts)
(e) PSI = 10% A3SS/(total transcripts)
(f) PSI = 8% A3SS/(total transcripts)
(g) PSI = 0% A3SS/(total transcripts)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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