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Gene Expression Data
Assay Details
Assay
Reference: J:198004 Xu J, et al., Notch2 is required in somatic cells for breakdown of ovarian germ-cell nests and formation of primordial follicles. BMC Biol. 2013;11:13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7564061
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: anti-Vasa
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 3C: postnatal day 3; Notch2tm3Grid/Notch2+ (more )
Note: Control littermate. Double labeled: orange - Ddx4; green - Amh (assay MGI:7564076).
Structure Level Pattern Image Note
TS27: oocyte of primordial follicle Present 3C Most oocytes expressing the cytoplasmic germ-cell marker Vasa in the cortical region of the ovary had been recruited into primordial follicles in the control littermate mice.
Specimen 3D: postnatal day 3; Amhr2tm3(cre)Bhr/Amhr2+, Notch2tm3Grid/Notch2tm3.1Grid (more )
Note: Conditional mutant. Double labeled: orange - Ddx4; green - Amh (assay MGI:7564076).
Structure Level Pattern Image Note
TS27: germ cell of ovary Present Regionally restricted 3D Immunopositive germ cells in the cortical region were still in germ-cell nests.
TS27: ovary cortex Present Regionally restricted 3D Immunopositive germ cells in the cortical region were still in germ-cell nests.
Specimen not shown E16.5 control: embryonic day 16.5; Notch2tm3Grid/Notch2+ (more )
Note: Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS24: oocyte Present Regionally restricted No oocytes in this period were double labeled with BrdU.
Specimen not shown E16.5 mutant: embryonic day 16.5; Amhr2tm3(cre)Bhr/Amhr2+, Notch2tm3Grid/Notch2tm3.1Grid (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS24: oocyte Present Regionally restricted As in the controls, no oocytes in this period were double labeled with BrdU in the mutant.
Specimen not shown E18.5 control: embryonic day 18.5; Notch2tm3Grid/Notch2+ (more )
Note: Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS26: oocyte Present Regionally restricted No oocytes in this period were double labeled with BrdU.
Specimen not shown E18.5 mutant: embryonic day 18.5; Amhr2tm3(cre)Bhr/Amhr2+, Notch2tm3Grid/Notch2tm3.1Grid (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS26: oocyte Present Regionally restricted As in the controls, no oocytes in this period were double labeled with BrdU in the mutant.
Specimen 5A: postnatal day 0; Notch2tm3Grid/Notch2+ (more )
Note: Control littermate. Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS27: oocyte Present Regionally restricted 5A Merge , 5A Vasa No oocytes in this period were double labeled with BrdU.
Specimen 5B: postnatal day 0; Amhr2tm3(cre)Bhr/Amhr2+, Notch2tm3Grid/Notch2tm3.1Grid (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS27: oocyte Present Regionally restricted 5B Merge , 5C , 5B Vasa As in the controls, no oocytes in this period were double labeled with BrdU in the mutant.
Specimen 6C: postnatal day 2; Notch2tm3Grid/Notch2+ (more )
Note: Control littermate. Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS27: oocyte Present Regionally restricted 6C Merge , 6C Vasa No oocytes in this period were double labeled with BrdU.
Specimen 6D: postnatal day 2; Amhr2tm3(cre)Bhr/Amhr2+, Notch2tm3Grid/Notch2tm3.1Grid (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - BrdU.
Structure Level Pattern Image Note
TS27: oocyte Present Regionally restricted 6D Vasa , 6E , 6D Merge As in the controls, no oocytes in this period were double labeled with BrdU in the mutant.
Specimen 7C: postnatal day 1; Notch2tm3Grid/Notch2+ (more )
Note: Control littermate. Double labeled: red - Ddx4; green - TUNEL.
Structure Level Pattern Image Note
TS27: oocyte Present 7C Merge , 7C Vasa Apoptotic oocytes were identified as immunopositive cells that were co-labeled with TUNEL.
Specimen 7D: postnatal day 1; Amhr2tm3(cre)Bhr/Amhr2+, Notch2tm3Grid/Notch2tm3.1Grid (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - TUNEL.
Structure Level Pattern Image Note
TS27: oocyte Present 7E , 7D Merge , 7D Vasa Significantly fewer apoptotic oocytes (immunopositive cells co-labeled with TUNEL) were detected in the mutant.
Specimen not shown Control P2: postnatal day 2; Notch2tm3Grid/Notch2+ (more )
Note: Double labeled: red - Ddx4; green - TUNEL.
Structure Level Pattern Image Note
TS27: oocyte Present Apoptotic oocytes were identified as immunopositive cells that were co-labeled with TUNEL.
Specimen not shown Mutant P2: postnatal day 2; Amhr2tm3(cre)Bhr/Amhr2+, Notch2tm3Grid/Notch2tm3.1Grid (more )
Note: Conditional mutant. Double labeled: red - Ddx4; green - TUNEL.
Structure Level Pattern Image Note
TS27: oocyte Present By P2, the numbers of apoptotic oocytes were not significantly different in mutant compared with control.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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