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Gene Expression Data
Assay Details
Assay
Reference: J:240101 Lv J, et al., 5-hydroxytryptamine synthesized in the aorta-gonad-mesonephros regulates hematopoietic stem and progenitor cell survival. J Exp Med. 2017 Feb;214(2):529-545
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7549028
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved caspase-3
Detection system: fluorescein
Results
Specimen 3C tph2+/+: embryonic day 10.5 (more )
Note: Triple labeled: red - Casp3; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 3C tph2+/+ (merged) , 3C tph2+/+ cCasp3
Specimen 3C tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Triple labeled: red - Casp3; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present Regionally restricted 3C tph2+/- (merged) , 3C tph2+/- cCasp3 Increased number of cells co-expressing both Pecam1 and CD34 compared to control
Specimen 3E pet1-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more )
Note: Triple labeled: red - Casp3; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 3E pet1-cre; tph2+/+ cCasp3 , 3E pet1-cre; tph2+/+ (merged)
Specimen 3E pet1-cre; tph2+/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Triple labeled: red - Casp3; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 3E pet1-cre; tph2+/fl cCasp3 , 3E pet1-cre; tph2+/fl (merged)
Specimen 3F tph2+/fl: embryonic day 10.5; Tph2tm1.1Kpl/Tph2+ (more )
Note: Double labeled: red - Casp3; green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 3F tph2+/fl
Specimen 3F vec-cre; tph2+/fl: embryonic day 10.5; Tg(Cdh5-cre)1Spe/0, Tph2tm1.1Kpl/Tph2+ (more )
Note: Conditional mutant. Double labeled: red - Casp3; green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 3F vec-cre; tph2+/fl
Specimen 6G tph2+/+: embryonic day 10.5 (more )
Note: Triple labeled: red - Casp3; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 6G tph2+/+ cCasp3 , 6G tph2+/+ (merged)
Specimen 6G tph2+/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2+ (more )
Note: Triple labeled: red - Casp3; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 6G tph2+/- cCasp3 , 6G tph2+/- (merged)
Specimen 6G tph2-/-: embryonic day 10.5; Tph2tm1.1Zfc/Tph2tm1.1Zfc (more )
Note: Triple labeled: red - Casp3; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).
Structure Level Pattern Image Note
TS17: dorsal aorta Present 6G tph2-/- cCasp3 , 6G tph2-/- (merged)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory