Cd34 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:240101 Lv J, et al., 5-hydroxytryptamine synthesized in the aorta-gonad-mesonephros regulates hematopoietic stem and progenitor cell survival. J Exp Med. 2017 Feb;214(2):529-545
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7549027
Gene symbol:	Cd34
Gene name:	CD34 antigen

Antibody:	anti-CD34
Detection system:	fluorescein

Results

2H pet1-cre; tph2+/+ ©

Specimen 2H pet1-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more

)
Note: Triple labeled: white - Cd34; green - Pecam1 (assay MGI:7549026); red - Runx1 (assay MGI:7549025).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		2H pet1-cre; tph2+/+

2H pet1-cre; tph2+/fl ©

Specimen 2H pet1-cre; tph2+/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2^tm1.1Kpl/Tph2⁺ (more

)
Note: Conditional mutant. Triple labeled: white - Cd34; green - Pecam1 (assay MGI:7549026); red - Runx1 (assay MGI:7549025).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		2H pet1-cre; tph2+/fl

3C tph2+/+ (merged) ©

3C tph2+/+ CD34 ©

Specimen 3C tph2+/+: embryonic day 10.5 (more

)
Note: Triple labeled: white - Cd34; red - Casp3 (assay MGI:7549028); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		3C tph2+/+ CD34 , 3C tph2+/+ (merged)

3C tph2+/- (merged) ©

3C tph2+/- CD34 ©

Specimen 3C tph2+/-: embryonic day 10.5; Tph2^tm1.1Zfc/Tph2⁺ (more

)
Note: Triple labeled: white - Cd34; red - Casp3 (assay MGI:7549028); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present	Regionally restricted	3C tph2+/- (merged) , 3C tph2+/- CD34	Increased number of cells co-expressing both Pecam1 and Casp3 compared to control

3E pet1-cre; tph2+/+ (merged) ©

3E pet1-cre; tph2+/+ CD34 ©

Specimen 3E pet1-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more

)
Note: Triple labeled: white - Cd34; red - Casp3 (assay MGI:7549028); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		3E pet1-cre; tph2+/+ CD34 , 3E pet1-cre; tph2+/+ (merged)

3E pet1-cre; tph2+/fl (merged) ©

3E pet1-cre; tph2+/fl CD34 ©

Specimen 3E pet1-cre; tph2+/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2^tm1.1Kpl/Tph2⁺ (more

)
Note: Conditional mutant. Triple labeled: white - Cd34; red - Casp3 (assay MGI:7549028); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		3E pet1-cre; tph2+/fl (merged) , 3E pet1-cre; tph2+/fl CD34	compared to control

4B E12.5 FL ©

Specimen 4B E12.5 FL: embryonic day 12.5 (more

)
Note: Triple labeled: white - Cd34; red - Fev (assay MGI:7549035); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS20: liver	Present		4B E12.5 FL

5B E10.5 AGM (merged) ©

5B E10.5 AGM CD34 ©

Specimen 5B E10.5 AGM: embryonic day 10.5 (more

)
Note: Triple labeled: white - Cd34; red - Htr5a (assay MGI:7549040); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: aorta-gonad-mesonephros	Present		5B E10.5 AGM (merged) , 5B E10.5 AGM CD34

5B E11.5 AGM (merged) ©

5B E11.5 AGM CD34 ©

Specimen 5B E11.5 AGM: embryonic day 11.5 (more

)
Note: Triple labeled: white - Cd34; red - Htr5a (assay MGI:7549040); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS19: aorta-gonad-mesonephros	Present		5B E11.5 AGM CD34 , 5B E11.5 AGM (merged)

5B E11.5 FL (merged) ©

5B E11.5 FL CD34 ©

Specimen 5B E11.5 FL: embryonic day 11.5 (more

)
Note: Triple labeled: white - Cd34; red - Htr5a (assay MGI:7549040); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS19: liver	Present		5B E11.5 FL CD34 , 5B E11.5 FL (merged)

Specimen 6D pet1-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more

)
Note: Triple labeled: white - Cd34; green - Pecam1 (assay MGI:7549026); red - Runx1 (assay MGI:7549025).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6D pet1-cre; tph2+/+

Specimen 6D pet1-cre; tph2fl/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2^tm1.1Kpl/Tph2^tm1.1Kpl (more

)
Note: Conditional mutant. Triple labeled: white - Cd34; green - Pecam1 (assay MGI:7549026); red - Runx1 (assay MGI:7549025).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6D pet1-cre; tph2fl/fl

Specimen 6G tph2+/+: embryonic day 10.5 (more

)
Note: Triple labeled: white - Cd34; green - Casp3 (assay MGI:7549028); red - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6G tph2+/+ CD34 , 6G tph2+/+ (merged)

Specimen 6G tph2+/-: embryonic day 10.5; Tph2^tm1.1Zfc/Tph2⁺ (more

)
Note: Triple labeled: white - Cd34; green - Casp3 (assay MGI:7549028); red - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6G tph2+/- (merged) , 6G tph2+/- CD34

Specimen 6G tph2-/-: embryonic day 10.5; Tph2^tm1.1Zfc/Tph2^tm1.1Zfc (more

)
Note: Triple labeled: white - Cd34; green - Casp3 (assay MGI:7549028); red - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6G tph2-/- CD34 , 6G tph2-/- (merged)

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