Runx1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:240101 Lv J, et al., 5-hydroxytryptamine synthesized in the aorta-gonad-mesonephros regulates hematopoietic stem and progenitor cell survival. J Exp Med. 2017 Feb;214(2):529-545
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7549025
Gene symbol:	Runx1
Gene name:	runt related transcription factor 1

Antibody:	anti-Runx1
Detection system:	fluorescein

Results

2B E10.5 tph2+/+ ©

Specimen 2B E10.5 tph2+/+: embryonic day 10.5 (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present	Regionally restricted	2B E10.5 tph2+/+	Expression in intraaortic hematopoietic clusters (IAHC).

2B E10.5 tph2+/- ©

Specimen 2B E10.5 tph2+/-: embryonic day 10.5; Tph2^tm1.1Zfc/Tph2⁺ (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Weak		2B E10.5 tph2+/-	Expression reduced compared to control.

2B E11.0 tph2 +/+ ©

Specimen 2B E11.0 tph2+/+: embryonic day 11.0 (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS18: dorsal aorta	Present	Regionally restricted	2B E11.0 tph2 +/+	Expression in intraaortic hematopoietic clusters (IAHC).

2B E11.0 tph2+/- ©

Specimen 2B E11.0 tph2+/-: embryonic day 11.0; Tph2^tm1.1Zfc/Tph2⁺ (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS18: dorsal aorta	Weak		2B E11.0 tph2+/-	Expression reduced compared to control.

2H pet1-cre; tph2+/+ ©

Specimen 2H pet1-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more

)
Note: Triple labeled: red - Runx1; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		2H pet1-cre; tph2+/+

2H pet1-cre; tph2+/fl ©

Specimen 2H pet1-cre; tph2+/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2^tm1.1Kpl/Tph2⁺ (more

)
Note: Conditional mutant. Triple labeled: red - Runx1; white - Cd34 (assay MGI:7549027); green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Weak		2H pet1-cre; tph2+/fl	Expression reduced compared to control.

2J E10.5 tph2+/fl ©

Specimen 2J E10.5 tph2+/fl: embryonic day 10.5; Tph2^tm1.1Kpl/Tph2⁺ (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		2J E10.5 tph2+/fl

2J E10.5 vec-cre; tph2+/fl ©

Specimen 2J E10.5 vec-cr; tph2+/fl: embryonic day 10.5; Tg(Cdh5-cre)1Spe/0, Tph2^tm1.1Kpl/Tph2⁺ (more

)
Note: Conditional mutant. Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Weak		2J E10.5 vec-cre; tph2+/fl	Expression reduced compared to control.

2J E11.0 tph2+/fl ©

Specimen 2J E11.0 tph2+/fl: embryonic day 11.0; Tph2^tm1.1Kpl/Tph2⁺ (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS18: dorsal aorta	Present		2J E11.0 tph2+/fl

2J E11.0 vec-cre; tph2+/fl ©

Specimen 2J E11.0 vec-cre; tph2+/fl: embryonic day 11.0; Tg(Cdh5-cre)1Spe/0, Tph2^tm1.1Kpl/Tph2⁺ (more

)
Note: Conditional mutant. Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS18: dorsal aorta	Weak		2J E11.0 vec-cre; tph2+/fl	Expression reduced compared to control.

3D tph2+/+ (merged) ©

3D tph2+/+ Runx1 ©

Specimen 3D tph2+/+: embryonic day 10.5 (more

)
Note: Triple labeled: white - Runx1; green - Pecam1 (assay MGI:7549026); red - TUNEL.

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		3D tph2+/+ Runx1 , 3D tph2+/+ (merged)

3D tph2+/- (merged) ©

3D tph2+/- Runx1 ©

Specimen 3D tph2+/-: embryonic day 10.5; Tph2^tm1.1Zfc/Tph2⁺ (more

)
Note: Triple labeled: white - Runx1; green - Pecam1 (assay MGI:7549026); red - TUNEL.

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present	Regionally restricted	3D tph2+/- (merged) , 3D tph2+/- Runx1	Authors report apoptotic nature of cells co-expressing Pecam1.

Specimen 6A E10.5 tph2+/+: embryonic day 10.5 (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6A E10.5 tph2+/+

Specimen 6A E10.5 tph2-/-: embryonic day 10.5; Tph2^tm1.1Zfc/Tph2^tm1.1Zfc (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6A E10.5 tph2-/-

Specimen 6A E11.0 tph2+/+: embryonic day 11.0 (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS18: dorsal aorta	Present		6A E11.0 tph2+/+

Specimen 6A E11.0 tph2-/-: embryonic day 11.0; Tph2^tm1.1Zfc/Tph2^tm1.1Zfc (more

)
Note: Double labeled: red - Runx1; green - Pecam1 (assay MGI:7549026).

Structure	Level	Pattern	Image	Note
TS18: dorsal aorta	Present		6A E11.0 tph2-/-

Specimen 6D pet1-cre; tph2+/+: embryonic day 10.5; Tg(Fev-cre)1Esd/0 (more

)

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6D pet1-cre; tph2+/+

Specimen 6D pet1-cre; tph2fl/fl: embryonic day 10.5; Tg(Fev-cre)1Esd/0, Tph2^tm1.1Kpl/Tph2^tm1.1Kpl (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present		6D pet1-cre; tph2fl/fl

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