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Gene Expression Data
Assay Details
Assay
Reference: J:340875 Zhou Z, et al., Fance deficiency inhibits primordial germ cell proliferation associated with transcription-replication conflicts accumulate and DNA repair defects. J Ovarian Res. 2023 Aug 10;16(1):160
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7544998
Gene symbol: Fut4
Gene name: fucosyltransferase 4
Antibody: Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A E11.5 +/+: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 1A E11.5 +/+ right , 1A E11.5 +/+ left Expressed in the cell membrane.
Specimen 1A E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 1A E11.5 -/- left , 1A E11.5 -/- right Positive cell number was significantly decreased compared with the wild-type (quantified in Fig 1B).
Specimen 1A E12.5 +/+: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 1A E12.5 +/+ right , 1A E12.5 +/+ left Expressed in the cell membrane.
Specimen 1A E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 1A E12.5 -/- right , 1A E12.5 -/- left Positive cell number was significantly decreased compared with the wild-type (quantified in Fig 1B).
Specimen 2A E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - BrdU.
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 2A E11.5 +/+ left , 2A E11.5 +/+ right
Specimen 2A E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - BrdU.
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 2A E11.5 -/- left , 2A E11.5 -/- right Decreased proportion in S phase (BrdU+Fut4+) compared with the wild-type (quantified in Fig 2C).
Specimen 2A E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - BrdU.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2A E12.5 +/+ left , 2A E12.5 +/+ right
Specimen 2A E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - BrdU.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2A E12.5 -/- left , 2A E12.5 -/- right Decreased proportion in S phase (BrdU+Fut4+) compared with the wild-type (quantified in Fig 2C).
Specimen 2B E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Ccnb1 (assay MGI:7545012).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 2B E11.5 +/+ left , 2B E11.5 +/+ right
Specimen 2B E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Ccnb1 (assay MGI:7545012).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 2B E11.5 -/- right , 2B E11.5 -/- left Decreased proportion in G2 phase (Ccnb1 positive in cytoplasm), and increased proportion in M phase (Ccnb1 positive in nucleus), compared with the wild-type (quantified in Fig 2C).
Specimen 2B E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Ccnb1 (assay MGI:7545012).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2B E12.5 +/+ left
Specimen 2B E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Ccnb1 (assay MGI:7545012).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2B E12.5 -/- left , 2B E12.5 -/- right Decreased proportion in G2 phase (Ccnb1 positive in cytoplasm), and increased proportion in M phase (Ccnb1 positive in nucleus), compared with the wild-type (quantified in Fig 2C).
Specimen 5A E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Lig1 (assay MGI:7545013).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5A E11.5 +/+ right , 5A E11.5 +/+ left
Specimen 5A E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Lig1 (assay MGI:7545013).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5A E12.5 -/- left , 5A E11.5 -/- right Colocalized with Lig1. No significant difference in the proportion of Lig1+ cells compared with the wild-type (quantified in Fig 5B).
Specimen 5A E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Lig1 (assay MGI:7545013).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 5A E12.5 +/+ right , 5A E12.5 +/+ left
Specimen 5A E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Lig1 (assay MGI:7545013).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 5A E12.5 -/- right , 5A E12.5 -/- left Colocalized with Lig1. No significant difference in the proportion of Lig1+ cells compared with the wild-type (quantified in Fig 5B).
Specimen 5C E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Pcna (assay MGI:7545014).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5C E11.5 +/+ right , 5C E11.5 +/+ left
Specimen 5C E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Pcna (assay MGI:7545014).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5C E11.5 -/- right , 5C E11.5 -/- left Colocalized with Pcna. No significant difference in the proportion of Pcna+ cells compared with the wild-type (quantified in Fig 5D).
Specimen 5C E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Pcna (assay MGI:7545014).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 5C E12.5 +/+ left , 5C E12.5 +/+ right
Specimen 5C E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Pcna (assay MGI:7545014).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 5C E12.5 -/- right , 5C E12.5 -/- left Colocalized with Pcna. No significant difference in the proportion of Pcna+ cells compared with the wild-type (quantified in Fig 5D).
Specimen 6A E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - S9.6 DNA-RNA Hybrid antibody.
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 6A E11.5 +/+ right , 6A E11.5 +/+ left
Specimen 6A E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - S9.6 DNA-RNA Hybrid antibody.
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 6A E11.5 -/- right , 6A E11.5 -/- left Co-localized staining of R-loop (transcription-replication conflicts, recognized by the S9.6 antibody) was shown. The proportion of R-loop positive cells was significantly increased compared with the wild-type (quantified in Fig 6B).
Specimen 6A E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - S9.6 DNA-RNA Hybrid antibody.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 6A E12.5 +/+ right , 6A E12.5 +/+ left
Specimen 6A E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - S9.6 DNA-RNA Hybrid antibody.
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 6A E12.5 -/- left , 6A E12.5 -/- right Co-localized staining of R-loop (transcription-replication conflicts, recognized by the S9.6 antibody) was shown. The proportion of R-loop positive cells was significantly increased compared with the wild-type (quantified in Fig 6B).
Specimen 7A E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Fancd2 (assay MGI:7545022).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 7A E11.5 +/+ right , 7A E11.5 +/+ left
Specimen 7A E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Fancd2 (assay MGI:7545022).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 7A E11.5 -/- left , 7A E11.5 -/- right Co-localized staining of Fancd2 was shown. The proportion of Fancd2 positive cells was significantly decreased compared with the wild-type (quantified in Fig 7B).
Specimen 7A E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Fancd2 (assay MGI:7545022).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 7A E12.5 +/+ left , 7A E12.5 +/+ right
Specimen 7A E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Fancd2 (assay MGI:7545022).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 7A E12.5 -/- left , 7A E12.5 -/- right Co-localized staining of Fancd2 was shown. The proportion of Fancd2 positive cells was significantly decreased compared with the wild-type (quantified in Fig 7B).
Specimen 8A E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Blm (assay MGI:7545025).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 8A E11.5 +/+ left , 8A E11.5 +/+ right
Specimen 8A E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Blm (assay MGI:7545025).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 8A E11.5 -/- left , 8A E11.5 -/- right The proportion of Blm positive cells was significantly decreased compared with the wild-type (quantified in Fig 8B).
Specimen 8A E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Blm (assay MGI:7545025).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 8A E12.5 +/+ right , 8A E12.5 +/+ left
Specimen 8A E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Blm (assay MGI:7545025).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 8A E12.5 -/- right , 8A E12.5 -/- left The proportion of Blm positive cells was significantly decreased compared with the wild-type (quantified in Fig 8B).
Specimen 8C E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Rad51 (assay MGI:7545027).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 8C E11.5 +/+ right , 8C E11.5 +/+ left
Specimen 8C E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Rad51 (assay MGI:7545027).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 8C E11.5 -/- right , 8C E11.5 -/- left The proportion of Rad51 positive cells was significantly decreased compared with the wild-type (quantified in Fig 8D).
Specimen 8C E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Rad51 (assay MGI:7545027).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 8C E12.5 +/+ left , 8C E12.5 +/+ right
Specimen 8C E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Rad51 (assay MGI:7545027).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 8C E12.5 -/- left , 8C E12.5 -/- right The proportion of Rad51 positive cells was significantly decreased compared with the wild-type (quantified in Fig 8D).
Specimen 9A E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Neil2 (assay MGI:7545030).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 9A E11.5 +/+ right , 9A E11.5 +/+ left
Specimen 9A E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Neil2 (assay MGI:7545030).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 9A E11.5 -/- left , 9A E11.5 -/- right The proportion of Neil2 positive cells was significantly decreased compared with the wild-type (quantified in Fig 9B).
Specimen 9A E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Neil2 (assay MGI:7545030).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 9A E12.5 +/+ right , 9A E12.5 +/+ left
Specimen 9A E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Neil2 (assay MGI:7545030).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 9A E12.5 -/- left , 9A E12.5 -/- right The proportion of Neil2 positive cells was significantly decreased compared with the wild-type (quantified in Fig 9B).
Specimen 9C E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: green - Fut4; red - Parp3 (assay MGI:7545029).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 9C E11.5 +/+ right , 9C E11.5 +/+ left
Specimen 9C E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Parp3 (assay MGI:7545029).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 9C E11.5 -/- left , 9C E11.5 -/- right There was no significant difference in the proportion of Parp3 positive cells compared with the wild-type (quantified in Fig 9D).
Specimen 9C E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: green - Fut4; red - Parp3 (assay MGI:7545029).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 9C E12.5 +/+ right , 9C E12.5 +/+ left
Specimen 9C E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: green - Fut4; red - Parp3 (assay MGI:7545029).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 9C E12.5 -/- right , 9C E12.5 -/- left There was no significant difference in the proportion of Parp3 positive cells compared with the wild-type (quantified in Fig 9D).
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