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Gene Expression Data
Assay Details
Assay
Reference: J:340875 Zhou Z, et al., Fance deficiency inhibits primordial germ cell proliferation associated with transcription-replication conflicts accumulate and DNA repair defects. J Ovarian Res. 2023 Aug 10;16(1):160
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7545012
Gene symbol: Ccnb1
Gene name: cyclin B1
Antibody: Anti-Cyclin B1 (4138)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2B E11.5 +/+: embryonic day 11.5 (more )
Note: Double labeled: red - Ccnb1; green - Fut4 (assay MGI:7544998).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 2B E11.5 +/+ right , 2B E11.5 +/+ left
Specimen 2B E11.5 -/-: embryonic day 11.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: red - Ccnb1; green - Fut4 (assay MGI:7544998).
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 2B E11.5 -/- right , 2B E11.5 -/- left Decreased proportion in G2 phase (Ccnb1 positive in cytoplasm), and increased proportion in M phase (Ccnb1 positive in nucleus), compared with the wild-type (quantified in Fig 2C).
Specimen 2B E12.5 +/+: embryonic day 12.5 (more )
Note: Double labeled: red - Ccnb1; green - Fut4 (assay MGI:7544998).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2B E12.5 +/+ right , 2B E12.5 +/+ left
Specimen 2B E12.5 -/-: embryonic day 12.5; FanceTg(Tyr)2364E-2a2Ove/FanceTg(Tyr)2364E-2a2Ove (more )
Note: Double labeled: red - Ccnb1; green - Fut4 (assay MGI:7544998).
Structure Level Pattern Image Note
TS20: primordial germ cell of gonad Present 2B E12.5 -/- left , 2B E12.5 -/- right Decreased proportion in G2 phase (Ccnb1 positive in cytoplasm), and increased proportion in M phase (Ccnb1 positive in nucleus), compared with the wild-type (quantified in Fig 2C).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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