Smad5 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:186504 Tanimoto Y, et al., Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2). J Biol Chem. 2012 Jun 15;287(25):21429-38
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7544957
Gene symbol:	Smad5
Gene name:	SMAD family member 5

Antibody:	anti-phospho-Smad1/5/9 (9511)
Detection system:	Biotinylated secondary antibody/[Strept]avidin
Assay notes:	The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; all family members have been annotated.

Results

7Da ©

Specimen 7Da: embryonic day 15.5 (more

)
Note: lambdoid suture

Structure	Level	Pattern	Image	Note
TS23: inter-parietal bone primordium	Strong	Regionally restricted	7Da	Expressed in osteogenic fronts in the nuclei.
TS23: parietal bone	Strong	Regionally restricted	7Da	Expressed in osteogenic fronts in the nuclei.
TS23: periosteum	Strong		7Da	Expressed in the nuclei.

7Db ©

Specimen 7Db: embryonic day 15.5; Gli3^Xt-J/Gli3^Xt-J (more

)
Note: lambdoid suture

Structure	Level	Pattern	Image	Note
TS23: chondrocranium	Strong	Regionally restricted	7Db	Ectopic expression in the mid-sutural mesenchymal cells throughout the suture area.

7Dc ©

Specimen 7Dc: embryonic day 15.5; Gli3^Xt-J/Gli3^Xt-J, Runx2^tm1Mjo/Runx2⁺ (more

)
Note: lambdoid suture

Structure	Level	Pattern	Image	Note
TS23: periosteum	Present		7Dc	Expressed in the nuclei, similar as in the wild-type.
TS23: inter-parietal bone primordium	Present	Regionally restricted	7Dc	Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
TS23: parietal bone	Present	Regionally restricted	7Dc	Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.

7Dd ©

Specimen 7Dd: embryonic day 15.5 (more

)
Note: coronal suture

Structure	Level	Pattern	Image	Note
TS23: frontal bone primordium	Strong	Regionally restricted	7Dd	Expressed in osteogenic fronts in the nuclei.
TS23: parietal bone	Strong	Regionally restricted	7Dd	Expressed in osteogenic fronts in the nuclei.
TS23: periosteum	Strong		7Dd	Expressed in the nuclei.

7De ©

Specimen 7De: embryonic day 15.5; Gli3^Xt-J/Gli3^Xt-J (more

)
Note: coronal suture

Structure	Level	Pattern	Image	Note
TS23: frontal bone primordium	Present	Regionally restricted	7De	Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
TS23: parietal bone	Present	Regionally restricted	7De	Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
TS23: periosteum	Present		7De	Expressed in the nuclei, similar as in the wild-type.

7Df ©

Specimen 7Df: embryonic day 15.5; Gli3^Xt-J/Gli3^Xt-J, Runx2^tm1Mjo/Runx2⁺ (more

)
Note: coronal suture

Structure	Level	Pattern	Image	Note
TS23: frontal bone primordium	Present	Regionally restricted	7Df	Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
TS23: parietal bone	Present	Regionally restricted	7Df	Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
TS23: periosteum	Present		7Df	Expressed in the nuclei, similar as in the wild-type.

7Dg ©

Specimen 7Dg: embryonic day 15.5 (more

)
Note: interfrontal suture

Structure	Level	Pattern	Image	Note
TS23: frontal bone primordium	Strong	Regionally restricted	7Dg	Expressed in osteogenic fronts in the nuclei.
TS23: periosteum	Strong		7Dg	Expressed in the nuclei.

7Dh ©

Specimen 7Dh: embryonic day 15.5; Gli3^Xt-J/Gli3^Xt-J (more

)
Note: interfrontal suture

Structure	Level	Pattern	Image	Note
TS23: chondrocranium	Strong	Regionally restricted	7Dh	Ectopic expression in the mid-sutural mesenchymal cells throughout the suture area.

7Di ©

Specimen 7Di: embryonic day 15.5; Gli3^Xt-J/Gli3^Xt-J, Runx2^tm1Mjo/Runx2⁺ (more

)
Note: interfrontal suture

Structure	Level	Pattern	Image	Note
TS23: periosteum	Present		7Di	Expressed in the nuclei, similar as in the wild-type.
TS23: frontal bone primordium	Present	Regionally restricted	7Di	Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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