Ncam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:337132 Hiremath C, et al., Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7539508
Gene symbol:	Ncam1
Gene name:	neural cell adhesion molecule 1

Antibody:	5B8 antibody
Detection system:	Secondary antibody

Results

Specimen 5A control: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz (more

)
Note: Triple labeled: green - Ncam1; white - Afdn (assay MGI:7539519); purple - Muc1 (assay MGI:7539509).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5A control right	Expressed at lateral cell junctions.

5A cKO right ©

Specimen 5A cKO: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Ncam1; white - Afdn (assay MGI:7539519); purple - Muc1 (assay MGI:7539509).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5A cKO right	Expressed at lateral cell junctions. Defects in lumen formation was seen.

Specimen S1 control: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz (more

)
Note: Double labeled: green - Ncam1; red - Pard6b (assay MGI:7539579).

Structure	Level	Pattern	Image	Note
TS22: nephron epithelium	Present		S1_control right

S1_cKO right ©

Specimen S1 cKO: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Ncam1; red - Pard6b (assay MGI:7539579).

Structure	Level	Pattern	Image	Note
TS22: nephron epithelium	Present		S1_cKO right

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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