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Gene Expression Data
Assay Details
Assay
Reference: J:337132 Hiremath C, et al., Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7539509
Gene symbol: Muc1
Gene name: mucin 1, transmembrane
Antibody: MUC1 Antibody (MH1 (CT2)) (MA5-11202)
Detection system: Secondary antibody
Results
Specimen 5A control: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Triple labeled: purple - Muc1; white - Afdn (assay MGI:7539519); green - Ncam1 (assay MGI:7539508).
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present Regionally restricted 5A control right Expressed at the apical surface.
Specimen 5A cKO: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: purple - Muc1; white - Afdn (assay MGI:7539519); green - Ncam1 (assay MGI:7539508).
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present Regionally restricted 5A cKO right Similar as in the controls, expressed at the apical surface.
Specimen 5B control 3D: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Double labeled: purple - Muc1; green - Cdh1 (assay MGI:7539507).
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present Regionally restricted 5B control 3D right Expressed at the apical surface.
TS22: renal vesicle Absent 5B control 3D right
Specimen 5B cKO 3D: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: purple - Muc1; green - Cdh1 (assay MGI:7539507).
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present Regionally restricted 5B cKO 3D right Similar as in the controls, expressed at the apical surface.
TS22: renal vesicle Absent 5B cKO 3D right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory