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Gene Expression Data
Assay Details
Assay
Reference: J:333559 Rupani DN, et al., Adar1 deletion causes degeneration of the exocrine pancreas via Mavs-dependent interferon signaling. Development. 2023 Jan 15;150(2):dev201097
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7539125
Gene symbol: Amy
Gene name: amylase complex
Antibody: anti-Amylase (3796)
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 2B Control: postnatal day 21; Adartm1Knk/?, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1a+/? (more )
Note: Multi-labeled: red - Amy; white - Cga (assay MGI:7539126); green - Krt19 (assay MGI:7539127); blue - hematoxylin. Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Cell Type Level Pattern Image Note
TS28: pancreatic acinus pancreatic acinar cell Present 2B Control , 2B Control inset
Specimen 2B CYA: postnatal day 21; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Multi-labeled: red - Amy; white - Cga (assay MGI:7539126); green - Krt19 (assay MGI:7539127); blue - hematoxylin.
Structure Cell Type Level Pattern Image Note
TS28: pancreas pancreatic acinar cell Weak 2B CYA inset , 2B CYA Authors report notable decreased in acinar cell area.
Specimen 2D Control: embryonic day 18.5; Adartm1Knk/?, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1a+/? (more )
Note: Triple labeled: green - Amy; yellow - Epcam (assay MGI:7539128); blue - DAPI. Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Level Pattern Image Note
TS26: pancreatic acinus Present 2D Control , 2D Control inset
Specimen 2D CYA: embryonic day 18.5; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Triple labeled: green - Amy; yellow - Epcam (assay MGI:7539128); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS26: pancreatic acinus pancreatic acinar cell Present Regionally restricted 2D CYA inset , 2D CYA Authors report increase in acinar lumen length.
Specimen 2E Control: postnatal day 2; Adartm1Knk/?, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1a+/? (more )
Note: Triple labeled: green - Amy; yellow - Epcam (assay MGI:7539128); blue - DAPI. Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Cell Type Level Pattern Image Note
TS27: pancreatic acinus pancreatic acinar cell Present 2E Control , 2E Control inset
Specimen 2E CYA: postnatal day 2; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Triple labeled: green - Amy; yellow - Epcam (assay MGI:7539128); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: pancreatic acinus pancreatic acinar cell Present Regionally restricted 2E CYA inset , 2E CYA Authors report increase in acinar lumen length.
TS27: gut Present Punctate 2E CYA inset , 2E CYA Authors report increased Amy-expressing puncta outside of pancreas.
Specimen 2I Control: embryonic day 18.5; Adartm1Knk/?, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1a+/? (more )
Note: Triple labeled: red - Amy; green - Sox9 (assay MGI:7539129); blue - hematoxylin. Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Cell Type Level Pattern Image Note
TS26: pancreatic acinus pancreatic acinar cell Present 2I Control , 2I Control inset
Specimen 2I CYA: embryonic day 18.5; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Triple labeled: red - Amy; green - Sox9 (assay MGI:7539129); blue - hematoxylin.
Structure Level Pattern Image Note
TS26: pancreas Present Regionally restricted 2I CYA inset , 2I CYA Increased number of cells co-expressing Amy and Sox9.
Specimen 2J Control: postnatal day 21; Adartm1Knk/?, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1a+/? (more )
Note: Multi-labeled: red - Amy; white - Krt19 (assay MGI:7539127); green - Sox9 (assay MGI:7539129); blue - hematoxylin. Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Cell Type Level Pattern Image Note
TS28: pancreatic acinus pancreatic acinar cell Present 2J Control inset , 2J Control
Specimen 2J CYA: postnatal day 21; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Multi-labeled: red - Amy; white - Krt19 (assay MGI:7539127); green - Sox9 (assay MGI:7539129); blue - hematoxylin.
Structure Level Pattern Image Note
TS28: pancreas Present Regionally restricted 2J CYA inset , 2J CYA Increased number of cells co-expressing Amy and Krt19 or Sox9.
Specimen S2B E18.5 Control: embryonic day 18.5; Adartm1Knk/?, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1a+/? (more )
Note: Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Cell Type Level Pattern Image Note
TS26: pancreatic acinus pancreatic acinar cell Present S2B E18.5 Control inset , S2B E18.5 Control
Specimen S2B E18.5 CYA: embryonic day 18.5; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: pancreas Present S2B E18.5 CYA , S2B E18.5 CYA inset Amy-positive area comparable to controls at E18.5
Specimen S2B P21 Control: postnatal day 21; Adartm1Knk/?, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1a+/? (more )
Note: Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Cell Type Level Pattern Image Note
TS28: pancreatic acinus pancreatic acinar cell Present S2B P21 Control inset , S2B P21 Control
Specimen S2B P21 CYA: postnatal day 21; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: pancreas Weak Regionally restricted S2B P21 CYA , S2B P21 CYA inset Amy-positive area drastically reduced at P21.
Specimen 4G Control: postnatal month 6; Adartm1Knk/Adar+, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Mavstm1Zjc/Mavstm1Zjc, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Multi-labeled: red - Amy; white - Cga (assay MGI:7539126); green - Krt19 (assay MGI:7539127); blue - hematoxylin. Control is either non-Cre-expressing OR Adar1 heterozygous litter mate.
Structure Cell Type Level Pattern Image Note
TS28: pancreatic acinus pancreatic acinar cell Present 4G Control , 4G Control inset
Specimen 4G CYAM: postnatal month 6; Adartm1Knk/Adartm1Knk, Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Mavstm1Zjc/Mavstm1Zjc, Ptf1atm1.1(cre)Cvw/Ptf1a+ (more )
Note: Conditional mutant. Multi-labeled: red - Amy; white - Cga (assay MGI:7539126); green - Krt19 (assay MGI:7539127); blue - hematoxylin.
Structure Cell Type Level Pattern Image Note
TS28: pancreatic acinus pancreatic acinar cell Present 4G CYAM inset , 4G CYAM No significant difference in acinar cells compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
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