Gene Expression Data
Assay Details
Assay
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Reference:
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J:249803
Guerrero-Flores G, et al., Functional determination of the differentiation potential of ventral mesencephalic neural precursor cells during dopaminergic neurogenesis. Dev Biol. 2017 Sep 1;429(1):56-70
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7538898
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Gene symbol:
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Lmx1a
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Gene name:
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LIM homeobox transcription factor 1 alpha
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Results
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Specimen
E9.5:
embryonic day 9.5
(more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Specimen
E9.5:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Data not shown. Cell counts were graphed in Figure 1A.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS15: future midbrain |
neural progenitor cell |
Present |
Regionally restricted |
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Expression was detected in the midbrain dopaminergic neural precursors.
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Specimen
E10.5:
embryonic day 10.5
(more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Specimen
E10.5:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Data not shown. Cell counts were graphed in Figure 1A.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS17: midbrain |
neural progenitor cell |
Present |
Regionally restricted |
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Expression was detected in the midbrain dopaminergic neural precursors.
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Specimen
E11.5:
embryonic day 11.5
(more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Specimen
E11.5:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Data not shown. Cell counts were graphed in Figure 1A.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: midbrain |
neural progenitor cell |
Present |
Regionally restricted |
|
Expression was detected in the midbrain dopaminergic neural precursors.
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Specimen
E12.0:
embryonic day 12.0
(more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Specimen
E12.0:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Data not shown. Cell counts were graphed in Figure 1A.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
neural progenitor cell |
Present |
Regionally restricted |
|
Expression was detected in the midbrain dopaminergic neural precursors.
|
Specimen
E12.5:
embryonic day 12.5
(more )
Note: Data not shown. Cell counts were graphed in Figure 1A.
Specimen
E12.5:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Data not shown. Cell counts were graphed in Figure 1A.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
neural progenitor cell |
Present |
Regionally restricted |
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Expression was detected in the midbrain dopaminergic neural precursors.
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Specimen
S1A:
embryonic day 12.5
(more )
Note: BrdU at E9.5. Double labeled: red - Lmx1a; green - BrdU.
Specimen
S1A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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BrdU at E9.5. Double labeled: red - Lmx1a; green - BrdU.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
midbrain dopaminergic neuron |
Present |
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S1A Lmx1a
,
S1A Merge top
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Few of the Lmx1a+ cells were double labeled with BrdU (injection at E9.5).
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Specimen
S1B:
embryonic day 12.5
(more )
Note: BrdU at E10.5. Triple labeled: blue - Lmx1a; red - Sox2 (assay MGI:7538886); green - BrdU.
Specimen
S1B:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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BrdU at E10.5. Triple labeled: blue - Lmx1a; red - Sox2 (assay MGI:7538886); green - BrdU.
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Specimen
S2A left:
embryonic day 10.5
(more )
Note: Double labeled: red - Lmx1a; green - Sox2 (assay MGI:7538886).
Specimen
S2A left:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Lmx1a; green - Sox2 (assay MGI:7538886).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: midbrain |
Present |
Regionally restricted |
S2A left
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Most cells in the Lmx1a+ domain were Sox2+.
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Specimen
S3A left:
embryonic day 11.5
(more )
Note: Double labeled: red - Lmx1a; green - Sox2 (assay MGI:7538886).
Specimen
S3A left:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Lmx1a; green - Sox2 (assay MGI:7538886).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: midbrain |
Present |
Regionally restricted |
S3A left
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Several Sox2+ cells were noted in the Lmx1a+ domain.
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