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Gene Expression Data
Assay Details
Assay
Reference: J:93831 Ebert SN, et al., Targeted insertion of the Cre-recombinase gene at the phenylethanolamine n-methyltransferase locus: A new model for studying the developmental distribution of adrenergic cells. Dev Dyn. 2004 Dec;231(4):849-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7536960
Gene symbol: Pnmt
Gene name: phenylethanolamine-N-methyltransferase
Antibody: anti-PNMT (AB110)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 2A: postnatal newborn; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pnmttm1(cre)Sne/Pnmt+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: adrenal medulla Present 2A
Specimen 2C: postnatal newborn; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pnmttm1(cre)Sne/Pnmt+ (more )
Note: Conditional mutant. Double labeled: green - Pnmt; blue - Pnmt reporter.
Structure Level Pattern Image Note
TS27: adrenal medulla Present 2C Costained with XGAL, although the XGAL blocked or quenched the fluorescence in costained cells.
Specimen 6C/D: embryonic day 9.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pnmttm1(cre)Sne/Pnmt+ (more )
Note: Conditional mutant. Overlay by merging the individual staining panels: green - Pnmt; red - Actn2 (assay MGI:7536966); blue - Pnmt reporter.
Structure Cell Type Level Pattern Image Note
TS15: atrium cardiac muscle cardiac muscle cell Present 6C , 6D Overlap with Actn2 satining.
TS15: bulbus cordis cardiac muscle cardiac muscle cell Present 6C , 6D Overlap with Actn2 satining.
TS15: primitive ventricle cardiac muscle cardiac muscle cell Present 6C , 6D Overlap with Actn2 satining.
TS15: trunk mesenchyme Present Regionally restricted 6D , 6C Pnmt+Actn2- cells were found just outside the heart, in both the anterior region adjacent to the outflow tract as well as in the posterior region adjacent to the atrial chamber.
Specimen 8E: postnatal day 2; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pnmttm1(cre)Sne/Pnmt+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: sinoatrial node Weak Regionally restricted 8E Expressed in a small cluster of cells.
Specimen not shown: postnatal (more )
Structure Level Pattern Image Note
TS28: adrenal medulla Present
Specimen not shown: postnatal; Pnmttm1(cre)Sne/Pnmt+ (more )
Structure Level Pattern Image Note
TS28: adrenal medulla Present
Specimen not shown: postnatal; Pnmttm1(cre)Sne/Pnmttm1(cre)Sne (more )
Structure Level Pattern Image Note
TS28: adrenal medulla Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory