Antibody:	anti-CTGF (ab6992)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Widespread	S1A CCN2	Expression detected in all organs, similar to that of Ccn5.
TS14: future brain	Present		S1A CCN2

Structure	Level	Pattern	Image	Note
TS16: embryo	Present	Widespread		Expression detected in all organs, similar to that of Ccn5.

Structure	Level	Pattern	Image	Note
TS18: embryo	Present	Widespread	S1B CCN2	Expression detected in all organs, similar to that of Ccn5.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		1a CCN2	Differences in expression from Ccn5 beginning at E12.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		1b CCN2	Expression in smaller number of cell types than Ccn5.

Structure	Level	Pattern	Image	Note
TS24: embryo	Present		1c CCN2	Expression in smaller number of cell types than Ccn5.

Structure	Level	Pattern	Image	Note
TS24: lung vascular element	Trace		2a CCN2	Very low levels of expression in veins and arteries.

Structure	Level	Pattern	Image	Note
TS20: heart apex	Strong		2b CCN2	Higher levels of expression in apex of heart when compared to myocardial expression levels.
TS20: cardiac muscle tissue	Present		2b CCN2

Structure	Level	Pattern	Image	Note
TS22: heart apex	Strong		2c CCN2	Higher levels of expression in apex of heart when compared to myocardial expression levels.
TS22: cardiac muscle tissue	Present		2c CCN2

Structure	Level	Pattern	Image	Note
TS22: heart apex	Strong		2d CCN2	Higher levels of expression in apex of heart when compared to myocardial expression levels.
TS22: cardiac muscle tissue	Present		2d CCN2
TS22: heart valve	Absent		2d CCN2

Structure	Level	Pattern	Image	Note
TS22: umbilical blood vessel	Present		2e CCN2

Structure	Level	Pattern	Image	Note
TS24: lung vascular element	Trace		S2A CCN2	Very low levels of expression in veins and arteries.

Structure	Level	Pattern	Image	Note
TS24: renal small blood vessel	Trace		S2B CCN2	Very low levels of expression in veins and arteries.

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Weak	Widespread	S2C CCN2	At E16, expression decreased throughout myocardium relative to expression at E14.

Structure	Level	Pattern	Image	Note
TS22: bronchiole	Very strong	Regionally restricted	3a CCN2	Very high expression at some terminal ends of actively branching bronchioles.

Structure	Level	Pattern	Image	Note
TS22: bronchiole epithelium	Present		3a CCN2 inset
TS22: lung mesenchyme	Absent		3a CCN2 inset	No expression in surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: bronchiole	Present	Regionally restricted	3b CCN2	Expression in actively branching broncholes branching from little to no expression to higher levels.

Structure	Level	Pattern	Image	Note
TS24: bronchus	Absent		3c CCN2	No expression in larger bronchi that are no longer branching.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Weak		4a CCN2	Very little expression in skeletal muscle. No accumulation of expresion at myotendinuous junctions.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: skeletal muscle		Weak		4b CCN2	Very little expression in skeletal muscle. No accumulation of expresion at myotendinuous junctions.
TS24: epiphyseal plate hypertrophic zone	growth plate cartilage chondrocyte	Present		4b CCN2	Expression in chondrocytes during endochondral ossification. Expression primarily in hypertrophic and calcification zones.
TS24: epiphyseal plate ossification zone	growth plate cartilage chondrocyte	Present		4b CCN2	Expression in chondrocytes during endochondral ossification. Expression primarily in hypertrophic and calcification zones.
TS24: epiphyseal plate resting zone		Absent		4b CCN2	No expression in hyaline cartilage in resting zone.
TS24: artery endothelium		Trace		4b CCN2	Very low levels of expression in veins and arteries.
TS24: artery smooth muscle		Trace		4b CCN2	Very low levels of expression in veins and arteries.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: rib	chondrocyte	Present	Regionally restricted	4c CCN2	Expression in chondrocytes during endochondral ossification.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: liver	hepatocyte	Present		5a CCN2
TS24: liver	hematopoietic stem cell	Absent		5a CCN2
TS24: liver	neutrophil	Absent		5a CCN2

Structure	Level	Pattern	Image	Note
TS20: small intestine epithelium	Strong		5b CCN2	Highly expressed in intestinal epithelium.
TS20: small intestine mesenchyme	Trace		5b CCN2	Very low expression in intestinal smooth muscle.

Structure	Level	Pattern	Image	Note
TS22: small intestine epithelium	Present		5c CCN2	Lower expression at E14.
TS22: intestine muscularis	Trace		5c CCN2	Very low expression in intestinal smooth muscle.

Structure	Level	Pattern	Image	Note
TS24: small intestine epithelium	Weak		5d CCN2	Much weaker expression by E16.
TS24: intestine muscularis	Trace		5d CCN2	Very low expression in intestinal smooth muscle.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	6a CCN2	Expression in metanephric tubules in tubule terminals rather than uniformly throughout tubules.

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present		6b CCN2	Expression only in tubules at E16.

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present		6c CCN2	Expression only in tubules at E16.

Structure	Level	Pattern	Image	Note
TS22: urothelium of bladder	Present		6d CCN2	Expression only in urothelium.

Structure	Level	Pattern	Image	Note
TS24: detrusor muscle of bladder	Present		6e CCN2	Expression in urothelium and smooth muscle.
TS24: urothelium of bladder	Present		6e CCN2	Expression in urothelium and smooth muscle.

Structure	Level	Pattern	Image	Note
TS22: ovary	Ambiguous		6f CCN2	Expression not described.

Structure	Level	Pattern	Image	Note
TS24: ovary	Weak		6g CCN2	Low levels of expression in ovary.
TS24: female accessory gland	Weak		6g CCN2	Low levels of expression in epithelial cells and glands of reproductive tract
TS24: female reproductive system epithelium	Weak		6g CCN2	Low levels of expression in epithelial cells and glands of reproductive tract

Structure	Level	Pattern	Image	Note
TS24: thyroid gland	Trace		7a CCN2	Very low levels of expression in thyroid gland.

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Present	Regionally restricted	7b CCN2	Expression in connective tissue of adrenal capsule.

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Present	Regionally restricted	7b CCN2 inset	Expression in nucleus of adrenal gland cells.

Structure	Level	Pattern	Image	Note
TS24: pancreatic duct	Weak			Low levels of expression in exocrine ducts.
TS24: spleen	Ambiguous			Absent or extremely low levels of expression in thymus and spleen.
TS24: thymus	Ambiguous			Absent or extremely low levels of expression in thymus and spleen.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	8a CCN2	Expression widely distributed in E12 brain.

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	8b CCN2	Expression more restricted at E14.
TS22: choroid plexus	Present		8b CCN2

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted	8c CCN2	Expression more restricted at E16.
TS24: choroid plexus	Present		8c CCN2

Structure	Cell Type	Level	Pattern	Image	Note
TS24: spinal cord	neuron	Strong	Regionally restricted	8d CCN2	Strong expression in nuclei of neurons.

Structure	Level	Pattern	Image	Note
TS24: vibrissa inner root sheath	Present		8e CCN2	Expression limitied to inner root sheath.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Strong		8f CCN2

Structure	Level	Pattern	Image	Note
TS24: eyelid	Present		8g CCN2	Expression in fused eyelid.
TS24: cornea epithelium	Present		8g CCN2
TS24: cornea stroma	Absent		8g CCN2