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Gene Expression Data
Assay Details
Assay
Reference: J:321979 Kurz J, et al., Notch signaling is a novel regulator of visceral smooth muscle cell differentiation in the murine ureter. Development. 2022 Feb 15;149(4):dev199735
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7531555
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 2C control: embryonic day 18.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Cell Type Level Pattern Image Note
TS26: ureteral smooth muscle layer ureter smooth muscle cell Present Regionally restricted 2C Acta2 control Expression was detected in smooth muscle cells of the tunica muscularis.
TS26: ureteral vasculature vascular associated smooth muscle cell Present Regionally restricted 2C Acta2 control Expression was detected in large adventitial vessels.
Specimen 2C Rbpj-cKO: embryonic day 18.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS26: ureteral smooth muscle layer ureter smooth muscle cell Present Regionally restricted 2C Acta2 Rbpj-cKO Expression was unchanged in the tunica muscularis of the mutant.
TS26: ureteral vasculature vascular associated smooth muscle cell Weak Regionally restricted 2C Acta2 Rbpj-cKO Expression was reduced in large adventitial vessels of the mutant.
Specimen 3B control: postnatal day 4; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Cell Type Level Pattern Image Note
TS28: ureteral smooth muscle layer smooth muscle cell Present 3B control Acta2
Specimen 3B Rbpj-cKO: postnatal day 4; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: ureteral smooth muscle layer smooth muscle cell Present 3B Rbpj-cKO Acta2 Expression was unchanged in the mutant compared with control.
Specimen 4B E14.5 control: embryonic day 14.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Level Pattern Image Note
TS22: ureteral mesenchyme Absent 4B control E14.5 Acta2
Specimen 4B E14.5 Rbpj-cKO: embryonic day 14.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: ureteral mesenchyme Absent 4B Rbpj-cKO E14.5 Acta2
Specimen 4B E15.5 control: embryonic day 15.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Level Pattern Image Note
TS23: ureteral mesenchyme Present 4B control E15.5 Acta2 Expression commenced at E15.5.
Specimen 4B E15.5 Rbpj-cKO: embryonic day 15.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: ureteral mesenchyme Weak 4B Rbpj-cKO E15.5 Acta2
Specimen 4B E16.5 control: embryonic day 16.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Level Pattern Image Note
TS24: ureteral mesenchyme Present 4B control E16.5 Acta2
Specimen 4B E16.5 Rbpj-cKO: embryonic day 16.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: ureteral mesenchyme Weak 4B Rbpj-cKO E16.5 Acta2
Specimen S3: embryonic day 18.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - lineage marker GFP.
Structure Cell Type Level Pattern Image Note
TS26: ureteral vasculature vascular associated smooth muscle cell Present S3_Acta2 red , S3_Acta2 merge , S3_Acta2 merge high mag Visceral and vascular Tbx18+ smooth muscle cells arise from mesenchymal progenitors.
TS26: ureteral mesenchyme ureter smooth muscle cell Present S3_Acta2 red , S3_Acta2 merge Visceral and vascular Tbx18+ smooth muscle cells arise from mesenchymal progenitors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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