Flg Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:240055 Grond S, et al., Skin Barrier Development Depends on CGI-58 Protein Expression during Late-Stage Keratinocyte Differentiation. J Invest Dermatol. 2017 Feb;137(2):403-413
Assay type:	Western blot
MGI Accession ID:	MGI:7529198
Gene symbol:	Flg
Gene name:	filaggrin

Antibody:

anti-filaggrin (PRB-417P; Poly19058)

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Genetic Background	Mutant Allele(s)	Sex	Note
flox/flox 1	E17.5	TS25: epidermis	Present	Present	Present	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze	Not Specified
flox/flox 2	E17.5	TS25: epidermis	Present	Present	Present	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze	Not Specified
epid-/- 1	E17.5	TS25: epidermis	Present (d)	Present (d)	Present (d)	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze, Tg(KRT14-cre)1Efu/0	Not Specified	Conditional mutant.
epid-/- 2	E17.5	TS25: epidermis	Present (d)	Present (d)	Present (d)	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze, Tg(KRT14-cre)1Efu/0	Not Specified	Conditional mutant.
flox/flox 1	E18.5	TS26: epidermis	Present	Present	Present	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze	Not Specified
flox/flox 2	E18.5	TS26: epidermis	Present	Present	Present	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze	Not Specified
epid-/- 1	E18.5	TS26: epidermis	Strong (e)	Strong (e)	Present (d)	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze, Tg(KRT14-cre)1Efu/0	Not Specified	Conditional mutant.
epid-/- 2	E18.5	TS26: epidermis	Strong (e)	Strong (e)	Present (d)	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze, Tg(KRT14-cre)1Efu/0	Not Specified	Conditional mutant.
flox/flox 1	P0	TS27: epidermis	Present	Present	Present	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze	Not Specified
flox/flox 2	P0	TS27: epidermis	Present	Present	Present	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze	Not Specified
epid-/- 1	P0	TS27: epidermis	Strong (e)	Strong (e)	Weak (f)	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze, Tg(KRT14-cre)1Efu/0	Not Specified	Conditional mutant.
epid-/- 2	P0	TS27: epidermis	Strong (e)	Strong (e)	Weak (f)	involves: 129P2/OlaHsd	Abhd5^tm1Rze/Abhd5^tm1Rze, Tg(KRT14-cre)1Efu/0	Not Specified	Conditional mutant.

Notes:
(a) 3FLG
(b) 2FLG
(c) 1FLG
(d) Similar as in the control.
(e) Increased abundance of multimeric, nonprocessed units compared with the control.
(f) Reduced abundance of active, monomeric units compared with the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24