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Gene Expression Data
Assay Details
Assay
Reference: J:339296 Fries M, et al., Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projections. Cell Rep. 2023 Aug 16;42(8):112985
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7525611
Gene symbol: Pou3f1
Gene name: POU domain, class 3, transcription factor 1
Antibody: anti-OCT6 (GTX134063)
Assay notes: Multiple Pou3f1 antibodies were described, but the one used in this assay was not detailed. Anti-POU3F1 (MABN738), MGI:7336054 , or anti-POU3F1 (#1909) MGI:7525389 or (#1894) MGI:7525390 may have been used in this assay.
Results
Specimen S1D POU3F1: postnatal adult (more )
Note: Triple labeled: green - Pou3f1; magenta - Chat (assay MGI:7525612); white - Gad2 (assay MGI:7525614).
Structure Cell Type Level Pattern Image Note
TS28: retina GABAergic amacrine cell Present S1D POU3F1 , S1D POU3F1/GAD6 , S1D POU3F1/ChAT Expression in (Gad2-positive) GABAergic amacrine cells.
TS28: retina amacrine cell Present Regionally restricted S1D POU3F1/GAD6 , S1D POU3F1 , S1D POU3F1/ChAT No expression in (Chat-positive) cholinergic amacrine cells.
Specimen 1A E15.5 POU3F1: embryonic day 15.5 (more )
Note: Triple labeled: green - Pou3f1; magenta - Pou4f1 (assay MGI:7525626); turquoise - Pou4f2 (assay MGI:7525628).
Structure Cell Type Level Pattern Image Note
TS23: retina retinal ganglion cell Present Regionally restricted 1A E15.5 (merged) , 1A E15.5 POU3F1 Among Pou4f1-expressing cells, 80% are retinal ganglion cells. 40% co-express both Pouf1 and Pou4f2.
Specimen 1A E17.5 POU3F1: embryonic day 17.5 (more )
Note: Triple labeled: green - Pou3f1; magenta - Pou4f1 (assay MGI:7525626); turquoise - Pou4f2 (assay MGI:7525628).
Structure Level Pattern Image Note
TS25: retina Present 1A E17.5 (merged) , 1A E17.5 POU3F1
Specimen 1A P0 POU3F1: postnatal day 0 (more )
Note: Triple labeled: green - Pou3f1; magenta - Pou4f1 (assay MGI:7525626); turquoise - Pou4f2 (assay MGI:7525628).
Structure Level Pattern Image Note
TS27: retina Present 1A P0 POU3F1 , 1A P0 (merged)
Specimen 1A Adult POU3F1: postnatal adult (more )
Note: Triple labeled: green - Pou3f1; magenta - Pou4f1 (assay MGI:7525626); turquoise - Pou4f2 (assay MGI:7525628).
Structure Level Pattern Image Note
TS28: retina Present 1A Adult POU3F1 , 1A Adult (merged)
Specimen 1F E14.5 POU3F1: embryonic day 14.5 (more )
Note: Double labeled: green - Pou3f1; magenta - Atoh7 (assay MGI:7525630).
Structure Level Pattern Image Note
TS22: retina Present 1F E14.5 POU3F1 , 1F E14.5 (merged) Only ~8% of expressing cells are co-labeled with Atoh7 at E14.5.
Specimen 1F E17.5 POU3F1: embryonic day 17.5 (more )
Note: Double labeled: green - Pou3f1; magenta - Atoh7 (assay MGI:7525630).
Structure Level Pattern Image Note
TS25: retina Present 1F E17.5 (merged) , 1F E17.5 POU3F1
Specimen 1F P0 POU3F1: postnatal day 0 (more )
Note: Double labeled: green - Pou3f1; magenta - Atoh7 (assay MGI:7525630).
Structure Level Pattern Image Note
TS27: retina Present 1F P0 (merged) , 1F P0 POU3F1
Specimen 1I POU3F1: embryonic day 16.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Slc6a4tm1(cre)Xz/Slc6a4+ (more )
Note: Conditional mutant. Double labeled: green - Pou3f1; magenta - recombinase reporter. Double labeled: green - Pou3f1; magenta - recombinase reporter.
Structure Level Pattern Image Note
TS24: retina Present Regionally restricted 1I POU3F1 , 1I POU3F1/TdTom Co expression with large proportion of TdTom-expressing cells in ventrotemporal area. Few expressing cells in TdTom+Pou4f2+ population.
Specimen S3A E13.5 POU3F1: embryonic day 13.5 (more )
Note: Double labeled: green - Pou3f1; magenta - EdU.
Structure Level Pattern Image Note
TS21: retina Present S3A E13.5 (merged) , S3A E13.5 POU3F1 Only 5% of expressing cells incorporate EdU following a 1h pulse.
Specimen S3A E15.5 POU3F1: embryonic day 15.5 (more )
Note: Double labeled: green - Pou3f1; magenta - EdU.
Structure Level Pattern Image Note
TS23: retina Present S3A E15.5 (merged) , S3A E15.5 POU3F1 Only 5% of expressing cells incorporate EdU following a 1h pulse.
Specimen S3A P0 POU3F1: postnatal day 0 (more )
Note: Double labeled: green - Pou3f1; magenta - EdU.
Structure Level Pattern Image Note
TS27: retina Present S3A P0 POU3F1 , S3A P0 (merged) Only 5% of expressing cells incorporate EdU following a 1h pulse.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
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