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Gene Expression Data
Assay Details
Assay
Reference: J:339296 Fries M, et al., Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projections. Cell Rep. 2023 Aug 16;42(8):112985
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7525628
Gene symbol: Pou4f2
Gene name: POU domain, class 4, transcription factor 2
Antibody: anti-BRN3B
Assay notes: Multiple Pou4f2 antibodies were described, but the one used in this assay was not detailed. Anti-BRN3B (600-101- MJ0) MGI:7525393 may have been used in this assay.
Results
Specimen 1A E15.5 BRN3B: embryonic day 15.5 (more )
Note: Triple labeled: turquoise - Pou4f2; green - Pou3f1 (assay MGI:7525611); magenta - Pou4f1 (assay MGI:7525626).
Structure Cell Type Level Pattern Image Note
TS23: retina retinal ganglion cell Present 1A E15.5 (merged) , 1A E15.5 BRN3B
Specimen 1A E17.5 BRN3B: embryonic day 17.5 (more )
Note: Triple labeled: turquoise - Pou4f2; green - Pou3f1 (assay MGI:7525611); magenta - Pou4f1 (assay MGI:7525626).
Structure Level Pattern Image Note
TS25: retina Present 1A E17.5 BRN3B , 1A E17.5 (merged)
Specimen 1A P0 BRN3B: postnatal day 0 (more )
Note: Triple labeled: turquoise - Pou4f2; green - Pou3f1 (assay MGI:7525611); magenta - Pou4f1 (assay MGI:7525626).
Structure Level Pattern Image Note
TS27: retina Present 1A P0 (merged) , 1A P0 BRN3B
Specimen 1A Adult BRN3B: postnatal adult (more )
Note: Triple labeled: turquoise - Pou4f2; green - Pou3f1 (assay MGI:7525611); magenta - Pou4f1 (assay MGI:7525626).
Structure Level Pattern Image Note
TS28: retina Present 1A Adult (merged) , 1A Adult BRN3B
Specimen 1I BRN3B (upper): embryonic day 16.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Slc6a4tm1(cre)Xz/Slc6a4+ (more )
Note: Conditional mutant. Double labeled: turquoise - Pou4f2; magenta - recombinase reporter.
Structure Level Pattern Image Note
TS24: retina Present Regionally restricted 1I Tdtom/BRN3B (upper) , 1I BRN3B (upper) Expression in ventrotemporal area.
Specimen 1I BRN3B (lower): embryonic day 16.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Slc6a4tm1(cre)Xz/Slc6a4+ (more )
Note: Conditional mutant. Double labeled: turquoise - Pou4f2; magenta - recombinase reporter.
Structure Level Pattern Image Note
TS24: retina Present Regionally restricted 1I BRN3B (lower) , 1I TdTom/BRN3B (lower) Expression in ventrotemporal area.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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