Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:229010 Wang J, et al., Yap and Taz play a crucial role in neural crest-derived craniofacial development. Development. 2016 Feb 1;143(3):504-15
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7518704
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Secondary antibody coupled to Cy3

Results

4A ©

Specimen 4A: embryonic day 10.5 (more

)
Note: Double labeled: red - Acta2; green - Pecam1 (assay MGI:7518687).

Structure	Level	Pattern	Image	Note
TS17: muscle tissue	Present	Regionally restricted	4A	Expression is in smooth muscle.

4B ©

Specimen 4B: embryonic day 10.5; Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno, H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺ (more

)
Note: Conditional mutant. Double labeled: red - Acta2; green - Pecam1 (assay MGI:7518687).

Structure	Level	Pattern	Image	Note
TS17: muscle tissue	Present	Regionally restricted	4B	Expression is in smooth muscle. This mutant had wrapped vessels around the hemangioma in the branchial arch.

6F ©

Specimen 6F: embryonic day 10.5 (more

)
Note: Double labeled: red - Acta2; green - Phospho-Histone 3.

Structure	Level	Pattern	Image	Note
TS17: muscle tissue	Present	Regionally restricted	6F	Expression is in smooth muscle.

6G ©

Specimen 6G: embryonic day 10.5 (more

)
Note: Double labeled: red - Acta2; green - Phospho-Histone 3.

Structure	Level	Pattern	Image	Note
TS17: muscle tissue	Present	Regionally restricted	6G	Expression is in smooth muscle.

6H ©

Specimen 6H: embryonic day 10.5; Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno, H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺ (more

)
Note: Conditional mutant. Double labeled: red - Acta2; green - Phospho-Histone 3.

Structure	Level	Pattern	Image	Note
TS17: muscle tissue	Present	Regionally restricted	6H	Expression is in smooth muscle.

6I ©

Specimen 6I: embryonic day 10.5; Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno, H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺ (more

)
Note: Conditional mutant. Double labeled: red - Acta2; green - Phospho-Histone 3.

Structure	Level	Pattern	Image	Note
TS17: muscle tissue	Present	Regionally restricted	6I	Expression is in smooth muscle.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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