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Gene Expression Data
Assay Details
Assay
Reference: J:332104 Company V, et al., Adhesion molecule Amigo2 is involved in the fasciculation process of the fasciculus retroflexus. Dev Dyn. 2022 Nov;251(11):1834-1847
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7514830
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Results
Specimen 1C: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: lateral habenular nucleus Present 1C
Specimen 1D: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 1D Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
Specimen 1E: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 1E Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
TS23: lateral habenular nucleus Present 1E
Specimen 1F: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 1F Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
TS23: midbrain Present 1F
TS23: pretectum Present 1F
TS23: thalamus Present 1F
Specimen 3A: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3A Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
TS23: lateral habenular nucleus Present 3A
TS23: pretectum Present 3A
TS23: thalamus Present 3A
Specimen 3B: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3B Expressed in segregated lateral habenular fasciculus retroflexus axons, accompanied all disaggregated tract portions generating corresponding shells in the mutant.
TS23: lateral habenular nucleus Present 3B
TS23: pretectum Present 3B
TS23: thalamus Present 3B
Specimen 3C: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3C Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
TS23: lateral habenular nucleus Present 3C
Specimen 3D: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3D Expressed in segregated lateral habenular fasciculus retroflexus axons, accompanied all disaggregated tract portions generating corresponding shells in the mutant.
TS23: lateral habenular nucleus Present 3D
Specimen 3E: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3E Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
Specimen 3F: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3F Expressed in segregated lateral habenular fasciculus retroflexus axons, accompanied all disaggregated tract portions generating corresponding shells in the mutant.
Specimen 4A: embryonic day 15.5 (more )
Note: Vibratome section. Level 1: at the initial segment located roughly 100 um away from the habenula. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4A Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
Specimen 4A': embryonic day 15.5 (more )
Note: Vibratome section. Level 2: at the middle of the dorsoventral course. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4A' Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
Specimen 4A": embryonic day 15.5 (more )
Note: Vibratome section. Level 3: at the final dorsoventral position, just before the defasciculation of the lateral habenular nucleus axons. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4A" Expressed in segregated lateral habenular fasciculus retroflexus axons, which placed in the sheath of the tract.
Specimen 4B: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 1: at the initial segment located roughly 100 um away from the habenula. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4B In a mild case, the tract was less compacted and generated unilateral aberrant duplications. Positive axons accompanied all disaggregated tract portions generating corresponding shells in the mutant.
Specimen 4B': embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 2: at the middle of the dorsoventral course. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4B' In a mild case, the tract was less compacted and generated unilateral aberrant duplications. Positive axons accompanied all disaggregated tract portions generating corresponding shells in the mutant.
Specimen 4B": embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 3: at the final dorsoventral position, just before the defasciculation of the lateral habenular nucleus axons. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4B" In a mild case, the tract was less compacted. Positive axons accompanied all disaggregated tract portions generating corresponding shells in the mutant.
Specimen 4C: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 1: at the initial segment located roughly 100 um away from the habenula. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4C In a severe case, the tract was much wider. Positive axons accompanied all disaggregated tract portions generating corresponding shells in the mutant.
Specimen 4C': embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 2: at the middle of the dorsoventral course. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4C' At this level, the tract was less compacted. Positive axons accompanied all disaggregated tract portions generating corresponding shells in the mutant.
Specimen 4C": embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 3: at the final dorsoventral position, just before the defasciculation of the lateral habenular nucleus axons. Double labeled: red - Nefm; green - Cntn2 (assay MGI:7514829).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4C" At this level, the tract was less compacted. Positive axons accompanied all disaggregated tract portions generating corresponding shells in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory