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Gene Expression Data
Assay Details
Assay
Reference: J:332104 Company V, et al., Adhesion molecule Amigo2 is involved in the fasciculation process of the fasciculus retroflexus. Dev Dyn. 2022 Nov;251(11):1834-1847
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7514829
Gene symbol: Cntn2
Gene name: contactin 2
Results
Specimen 1C: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: medial habenular nucleus Present 1C

Specimen 1D: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 1D Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 1E: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 1E Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 1F: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 1F Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 3A: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3A Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 3B: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present 3B The tract was wide, and even divided in two fascicles in the mutant embryos and the axons were less fasciculated.

Specimen 3C: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3C Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.
TS23: medial habenular nucleus Present 3C

Specimen 3D: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3D The tract was wide, and even divided in two fascicles in the mutant embryos and the axons were less fasciculated.
TS23: medial habenular nucleus Present 3D

Specimen 3E: embryonic day 15.5 (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3E Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 3F: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 3F The tract was wide, and even divided in two fascicles in the mutant embryos.

Specimen 4A: embryonic day 15.5 (more )
Note: Vibratome section. Level 1: at the initial segment located roughly 100 um away from the habenula. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4A Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 4A': embryonic day 15.5 (more )
Note: Vibratome section. Level 2: at the middle of the dorsoventral course. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4A' Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 4A": embryonic day 15.5 (more )
Note: Vibratome section. Level 3: at the final dorsoventral position, just before the defasciculation of the lateral habenular nucleus axons. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4A" Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core.

Specimen 4B: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 1: at the initial segment located roughly 100 um away from the habenula. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4B Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core. In a mild case, the tract was less compacted and generated unilateral aberrant duplications.

Specimen 4B': embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 2: at the middle of the dorsoventral course. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4B' Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core. In a mild case, the tract was less compacted and generated unilateral aberrant duplications.

Specimen 4B": embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 3: at the final dorsoventral position, just before the defasciculation of the lateral habenular nucleus axons. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4B" Expressed in segregated medial habenular fasciculus retroflexus axons, which placed tightly in the core. In a mild case, the tract was less compacted.

Specimen 4C: embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 1: at the initial segment located roughly 100 um away from the habenula. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4C In a severe case, the tract was much wider. Positive axons stayed in the core.

Specimen 4C': embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 2: at the middle of the dorsoventral course. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4C' In a severe case, the tract was much wider. Positive axons stayed in the core.

Specimen 4C": embryonic day 15.5; Amigo2tm1Lti/Amigo2tm1Lti (more )
Note: Vibratome section. Level 3: at the final dorsoventral position, just before the defasciculation of the lateral habenular nucleus axons. Double labeled: green - Cntn2; red - Nefm (assay MGI:7514830).
Structure Level Pattern Image Note
TS23: fasciculus retroflexus Present Regionally restricted 4C" In a severe case, the tract was much wider. Positive axons stayed in the core.


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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory