Sall1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:161758 Kiefer SM, et al., SALL1 truncated protein expression in Townes-Brocks syndrome leads to ectopic expression of downstream genes. Hum Mutat. 2008 Sep;29(9):1133-40
Assay type:	Northern blot
MGI Accession ID:	MGI:7514260
Gene symbol:	Sall1
Gene name:	spalt like transcription factor 1

Probe:	Sall1 cDNA
Probe preparation:	Double stranded labelled with digoxigenin DNA
Visualized with:	Alkaline phosphatase

Results

Image: 3B

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	5 kb (a)	3.4 kb (b)	3.1 kb (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	E12.5	TS20: limb	Present	Absent	Absent	10 µg; total RNA	Not Specified		Not Specified
delta/+	E12.5	TS20: limb	Present	Absent	Present	10 µg; total RNA	involves: 129X1/SvJ	Sall1^tm1Mrau/Sall1⁺	Not Specified
delta-cre/+	E12.5	TS20: limb	Present	Present	Absent	10 µg; total RNA	involves: 129X1/SvJ	Sall1^tm1.1Mrau/Sall1⁺	Not Specified

Notes:
(a) This represents the wild-type transcript.
(b) This represents the transcirpt spliced to exon III after cassette removal by cre.
(c) This represents the transcript that terminates after the neomycin cassette in exon II.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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