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Gene Expression Data
Assay Details
Assay
Reference: J:337240 Huang X, et al., Generation and characterization of a Myh6-driven Cre knockin mouse line. Transgenic Res. 2021 Dec;30(6):821-835
Assay type: Recombinase reporter
MGI Accession ID: MGI:7511416
Gene symbol: Myh6
Gene name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Reporter: tdTomato
Antibody: anti-tdTomato (600-401-379)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen Fig2(b): embryonic day 9.5; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS15: heart cardiac muscle cell Strong 2(b) left , 2(b) right overlaps with anti-TNNI3 immunopositive cell
Specimen Fig2(e): embryonic day 13.5; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: heart cardiac muscle cell Present 2(e) middle , 2(e) left overlaps with anti-TNNI3 immunopositive cell
Specimen Fig2(g): embryonic day 13.5; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: heart cardiac endothelial cell Absent 2(g) left , 2(g) middle not in anti-CDH5 immunopositive endothelial cells
Specimen Fig3(c): postnatal week 2; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cardiac muscle tissue cardiac muscle cell Present Uniform 3(c) right , 3(c) left overlaps with anti-TNNI3 immunopositive cell
Specimen Fig3(d): postnatal week 2; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: heart fibroblast of cardiac tissue Absent 3(d) left , 3(d) right not in anti-PDFGRa immunopositive cardial fibroblast cells
Specimen Fig3(e): postnatal week 2; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: heart cardiac endothelial cell Absent 3(e) right , 3(e) left not in anti-CDH5 immunopositive endothelial cells; not in anti-PDGFRb immunopositive coronary vascular pericyte
TS28: heart pericyte Absent 3(e) right , 3(e) left not in anti-CDH5 immunopositive endothelial cells; not in anti-PDGFRb immunopositive coronary vascular pericyte
Specimen Fig3(f): postnatal week 2; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: heart smooth muscle cell Absent 3(f) right , 3(f) left not in anti-aSMA immunopositive smooth muscle cells
Specimen Fig4(c): postnatal week 8; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: cardiac muscle tissue cardiac muscle myoblast Present 4(c) overlaps with anti-TNNI3 immunopositive cell
Specimen Fig4(d): postnatal week 8; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: heart fibroblast of cardiac tissue Absent 4(d) not in anti-PDFGRa immunopositive cardial fibroblast cells
Specimen Fig4(e): postnatal week 8; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: heart cardiac endothelial cell Absent 4(e) not in anti-CDH5 immunopositive endothelial cells; not in anti-PDGFRb immunopositive coronary vascular pericyte
TS28: heart pericyte Absent 4(e) not in anti-CDH5 immunopositive endothelial cells; not in anti-PDGFRb immunopositive coronary vascular pericyte
Specimen Fig4(f): postnatal week 8; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: coronary artery blood vessel smooth muscle cell Present Single cells 4(f) in a few cells of anti-aSMA immunopositive smooth muscle cells in coronary vascularature
Specimen Fig5: postnatal week 8; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Myh6em2(cre)Smoc/Myh6+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: liver Present Single cells 5(a) LIver DAPI tdTomato only a few positive cells
TS28: thymus Present Single cells 5(a) Thymus DAPI tdTomato only a few positive cells
TS28: metanephros Present 5(a) Kidney DAPI tdTomato
TS28: spleen Present 5(a) Spleen DAPI tdTomato
TS28: skeletal muscle Moderate 5(a) Skeletal muscle DAPI tdTomato
TS28: lung Present 5(a) Lung DAPI tdTomato
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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