Probe:	Lrrk2 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme	Present		1C/C'
TS16: urogenital ridge	Present		1C/C'

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme	Present		1D/D'
TS16: urogenital ridge	Present		1D/D'

Structure	Level	Pattern	Image	Note
TS20: meninges	Strong		1F/F'
TS20: choroid plexus	Present		1F/F'
TS20: pituitary gland	Present		1F/F'

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present		1H/H'
TS23: heart	Present		1H/H'
TS23: liver	Present		1H/H'
TS23: lung	Present		1H/H'
TS23: metanephros	Present		1H/H'

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2A/A'
TS28: corpus striatum	Present		2A/A'
TS28: olfactory tubercle	Present		2A/A'

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2B/B'
TS28: hippocampus	Present		2B/B'
TS28: substantia nigra pars compacta	Weak		2B/B'

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Moderate		2C/C'
TS28: olfactory tubercle	Moderate		2C/C'
TS28: cerebral cortex	Present		2C/C'

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Moderate		2D/D'
TS28: hippocampus	Moderate		2D/D'
TS28: substantia nigra pars compacta	Weak		2D/D'
TS28: substantia nigra pars reticulata	Weak		2D/D'
TS28: ventral tegmental area	Weak		2D/D'

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong		2E/E'
TS28: olfactory tubercle	Strong		2E/E'
TS28: cerebral cortex	Present		2E/E'

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong		2F/F'
TS28: hippocampus	Strong		2F/F'
TS28: substantia nigra pars compacta	Weak		2F/F'
TS28: substantia nigra pars reticulata	Weak		2F/F'
TS28: ventral tegmental area	Weak		2F/F'

Structure	Level	Pattern	Image	Note
TS28: brain	Strong	Regionally restricted	3D/D'	Expression is in various regions throughout the brain.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Very strong	Regionally restricted	3E/E'
TS28: forebrain	Very strong	Regionally restricted	3E/E'
TS28: olfactory tubercle	Very strong	Regionally restricted	3E/E'
TS28: CA1 pyramidal cell layer	Strong		3E/E'
TS28: CA2 pyramidal cell layer	Strong		3E/E'
TS28: CA3 pyramidal cell layer	Strong		3E/E'
TS28: anterior olfactory nucleus	Strong		3E/E'
TS28: cerebellum Purkinje cell layer	Strong		3E/E'
TS28: cerebellum molecular layer	Strong		3E/E'
TS28: dentate gyrus	Strong		3E/E'
TS28: prefrontal cortex	Strong		3E/E'
TS28: somatosensory cortex	Strong		3E/E'
TS28: substantia nigra pars compacta	Weak		3E/E'
TS28: substantia nigra pars reticulata	Weak		3E/E'
TS28: ventral tegmental area	Weak		3E/E'

Structure	Cell Type	Level	Pattern	Image	Note
TS28: corpus striatum	neuron	Present		5A

Structure	Level	Pattern	Image	Note
TS11: yolk sac	Strong	Regionally restricted	S1B/B'	Expression is in the parietal yolk sac.
TS11: embryo ectoderm	Absent		S1B/B'
TS11: embryo endoderm	Absent		S1B/B'
TS11: embryo mesoderm	Absent		S1B/B'

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong		S1B/B'

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Strong		S1C/C'
TS22: heart	Strong		S1C/C'
TS22: liver	Strong		S1C/C'
TS22: lung	Strong		S1C/C'
TS22: metanephros	Strong		S1C/C'
TS22: nasal cartilage	Strong		S1C/C'

Structure	Level	Pattern	Image	Note
TS22: lower jaw incisor	Strong		S1D/D'
TS22: upper jaw incisor	Strong		S1D/D'

Structure	Level	Pattern	Image	Note
TS22: nephron	Strong		S1E/E'

Structure	Level	Pattern	Image	Note
TS27: choroid plexus	Strong		S2A/A'
TS27: brainstem	Weak		S2A/A'
TS27: cerebellum	Weak		S2A/A'
TS27: cerebral cortex	Weak		S2A/A'

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Strong		S2B/B'
TS28: cerebral cortex	Present		S2B/B'
TS28: corpus striatum	Present		S2B/B'
TS28: main olfactory bulb	Present		S2B/B'

Structure	Level	Pattern	Image	Note
TS28: visual cortex	Strong	Regionally restricted	S3B/B'	Expression is in cortical layers 2 to 5a.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong		S4B

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present		S4D	Expression is in ependymal cells as well as in deeper layers.
TS28: choroid plexus	Present		S4D

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Strong		S4F
TS28: CA2 pyramidal cell layer	Strong		S4F
TS28: CA3 pyramidal cell layer	Strong		S4F
TS28: dentate gyrus	Strong		S4F
TS28: hippocampus	Present		S4F

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Weak		S4H
TS28: substantia nigra pars reticulata	Weak		S4H

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		S4J
TS28: cerebellum molecular layer	Strong		S4J

Structure	Level	Pattern	Image	Note
TS14: embryo	Weak	Regionally restricted		The authors state that weak expression was found over the entire embryo from E9.0-E11.5 beside the neuroepithelium of the central nervous system.
TS14: central nervous system	Absent

Structure	Level	Pattern	Image	Note
TS19: embryo	Weak	Regionally restricted		The authors state that weak expression was found over the entire embryo from E9.0-E11.5 beside the neuroepithelium of the central nervous system.
TS19: central nervous system	Absent

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis	Present
TS28: neurohypophysis	Absent

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted		The authors state that the expression patterns and levels were maintained up to 24 month old mice.