Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:103991
Ijiri K, et al., A novel role for GADD45beta as a mediator of MMP-13 gene expression during chondrocyte terminal differentiation. J Biol Chem. 2005 Nov 18;280(46):38544-55
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7506320
|
|
Gene symbol:
|
Mmp13
|
|
Gene name:
|
matrix metallopeptidase 13
|
|
Probe:
|
Mmp13 cDNA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
3H:
embryonic day 15.5
(more  )
Specimen
3H:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
3H
|
Expression is in late stage hypertrophic chondrocytes.
|
Specimen
3K:
embryonic day 15.5
(more )
Specimen
3K:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
3K
|
Expression is in late stage hypertrophic chondrocytes.
|
Specimen
4C:
embryonic day 15.5
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
4C
|
Expression is in hypertrophic chondrocytes.
|
Specimen
4F:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
|
Mutant Allele(s):
|
Gadd45btm1Flv/Gadd45btm1Flv
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
hypertrophic chondrocyte |
Weak |
Regionally restricted |
4F
|
In this mutant the number and distribution of expressing hypertrophic chondrocytes was reduced compared to wild-type.
|
Specimen
4G:
embryonic day 15.5
(more )
Specimen
4G:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
4G
|
Expression is in hypertrophic chondrocytes.
|
Specimen
4H:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
|
Mutant Allele(s):
|
Gadd45btm1Flv/Gadd45btm1Flv
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
hypertrophic chondrocyte |
Weak |
Regionally restricted |
4H
|
In this mutant the number and distribution of expressing hypertrophic chondrocytes was reduced compared to wild-type.
|
Specimen
5E WT:
embryonic day 16.5
(more )
Specimen
5E WT:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
5E WT Mmp13
|
Expression is in hypertrophic chondrocytes.
|
Specimen
5E Gadd45b<-/->:
(close )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
|
Mutant Allele(s):
|
Gadd45btm1Flv/Gadd45btm1Flv
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
hypertrophic chondrocyte |
Weak |
Regionally restricted |
5E Gadd45b-/- Mmp13
|
In this mutant the number and distribution of expressing hypertrophic chondrocytes was reduced compared to wild-type.
|
|
Analysis Tools