Gene Expression Data
Assay Details
Assay
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Reference:
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J:103991
Ijiri K, et al., A novel role for GADD45beta as a mediator of MMP-13 gene expression during chondrocyte terminal differentiation. J Biol Chem. 2005 Nov 18;280(46):38544-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7506320
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Gene symbol:
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Mmp13
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Gene name:
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matrix metallopeptidase 13
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Probe:
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Mmp13 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3H:
embryonic day 15.5
(more )
Specimen
3H:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
3H
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Expression is in late stage hypertrophic chondrocytes.
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Specimen
3K:
embryonic day 15.5
(more )
Specimen
3K:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
3K
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Expression is in late stage hypertrophic chondrocytes.
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Specimen
4C:
embryonic day 15.5
(more )
Specimen
4C:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
4C
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Expression is in hypertrophic chondrocytes.
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Specimen
4F:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Gadd45btm1Flv/Gadd45btm1Flv
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: tibia |
hypertrophic chondrocyte |
Weak |
Regionally restricted |
4F
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In this mutant the number and distribution of expressing hypertrophic chondrocytes was reduced compared to wild-type.
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Specimen
4G:
embryonic day 15.5
(more )
Specimen
4G:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
4G
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Expression is in hypertrophic chondrocytes.
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Specimen
4H:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Gadd45btm1Flv/Gadd45btm1Flv
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: tibia |
hypertrophic chondrocyte |
Weak |
Regionally restricted |
4H
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In this mutant the number and distribution of expressing hypertrophic chondrocytes was reduced compared to wild-type.
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Specimen
5E WT:
embryonic day 16.5
(more )
Specimen
5E WT:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: tibia |
hypertrophic chondrocyte |
Present |
Regionally restricted |
5E WT Mmp13
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Expression is in hypertrophic chondrocytes.
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Specimen
5E Gadd45b<-/->:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Gadd45btm1Flv/Gadd45btm1Flv
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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Not Specified
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: tibia |
hypertrophic chondrocyte |
Weak |
Regionally restricted |
5E Gadd45b-/- Mmp13
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In this mutant the number and distribution of expressing hypertrophic chondrocytes was reduced compared to wild-type.
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