About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:275851 Harris C, et al., Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2. Elife. 2019 Apr 2;8:e44258
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7495828
Gene symbol: Eed
Gene name: embryonic ectoderm development
Antibody: anti-EED
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1A female: embryonic day 3.5; Tg(CAG-EGFP)D4Nagy/0 (more )
Note: Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
Structure Level Pattern Image Note
TS4: blastocyst Present 1A EED female , 1A lower EED female Accumulation of Eed, H3K27me3 with Xist RNA in a vast majority of the nuclei (72-100%).
Specimen 1A male: embryonic day 3.5 (more )
Note: Tg(CAG-EGFP)D4Nagy was used to sex the embryo.
Structure Level Pattern Image Note
TS4: blastocyst Absent 1A EED male , 1A lower EED male
Specimen 1B +/-: embryonic day 3.0-3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 1B +/- lower EED , 1B +/- EED Coincident accumulation of Eed and/or H3K27me3 with Xist RNA in over 70% of the nuclei (9 out of 41 female embryos), similar as in the wild-type female.
Specimen 1B -/-: embryonic day 3.0-3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Absent 1B -/- EED , 1B -/- lower EED Devoid of Eed or H3K27me3 enrichment overlapping with the Xist RNA coat (9 out of 41 female embryos).
Specimen not shown: embryonic day 3.0-3.5; Eedtm1d(EUCOMM)Wtsi/? (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 23 out of 41 female embryos displayed 2-70% of nuclei with Eed and/or H3K27me3 enrichment, likely representing Eed+/- or Eed-/- embryos that had not yet fully depleted maternally-inherited Eed protein or Eed+/- embryos which had not yet robustly expressed zygotic Eed.
Specimen not shown: embryonic day 3.0-3.5; Eedtm1d(EUCOMM)Wtsi/? (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline.
Structure Level Pattern Image Note
TS4: blastocyst Absent No enrichment of Eed or H3K27me3 in the nucleus, as in the WT male embryos.
Specimen 2A 2-cell fl/fl: embryonic day 1.0; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Note: Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 2A fl/fl
Specimen 2A 2-cell fl/- or -/-: embryonic day 1.0; Eedtm1d(EUCOMM)Wtsi/? (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 2A fl/- or -/- Exhibited similar levels of Eed and H3K27me3 as in the control (summarized in 2C and 2E).
Specimen 2A 2-cell m-/-: embryonic day 1.0; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 2A m-/- Devoid of both Eed and H3K27me3 (summarized in 2C and 2E).
Specimen 2A 2-cell mz-/-: embryonic day 1.0; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 2A mz-/- Devoid of both Eed and H3K27me3 (summarized in 2C and 2E).
Specimen 2-S1B 4-cell fl/fl: embryonic day 2.0; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Note: Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 2-S1B 4-cell fl/fl
Specimen 2-S1B 4-cell fl/- or -/-: embryonic day 2.0; Eedtm1d(EUCOMM)Wtsi/? (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 2-S1B 4-cell fl/- or -/- A subset of embryos displayed reduced Eed and H3K27me3 levels (summarized in 2C and 2E).
Specimen 2-S1B 4-cell m-/-: embryonic day 2.0; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Trace 2-S1B 4-cell m-/- Zygotic Eed transcription initiated (summarized in 2C and 2E).
Specimen 2-S1B 4-cell mz-/-: embryonic day 2.0; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 2-S1B 4-cell mz-/-
Specimen 2-S1B 8-cell fl/fl: embryonic day 2.5; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Note: Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2-S1B 8-cell fl/fl
Specimen 2-S1B 8-cell fl/- or -/-: embryonic day 2.5; Eedtm1d(EUCOMM)Wtsi/? (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2-S1B 8-cell fl/- or -/- Highly variable Eed and H3K27me3 levels, suggesting further differentiation of the two genotypes (summarized in 2C and 2E).
Specimen 2-S1B 8-cell m-/-: embryonic day 2.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Weak 2-S1B 8-cell m-/- In agreement with increasing zygotic Eed expression, Eed m-/- embryos displayed higher levels of Eed and H3K27me3 than the corresponding Eed mz-/- embryos (summarized in 2C and 2E).
Specimen 2-S1B 8-cell mz-/-: embryonic day 2.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2-S1B 8-cell mz-/-
Specimen 2B 16-cell fl/fl: embryonic day 3.0; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Note: Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS4: compacted morula Present 2B fl/fl
Specimen 2B 16-cell fl/-: embryonic day 3.0; Eedtm1c(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS4: compacted morula Present 2B fl/- Statistically indistinguishable from Eed fl/fl embryos (summarized in 2C and 2E).
Specimen 2B 16-cell -/-: embryonic day 3.0; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS4: compacted morula Present 2B -/- Expressed at a lower levels compared with the Eed fl/fl controls (summarized in 2C and 2E).
Specimen 2B 16-cell m-/-: embryonic day 3.0; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS4: compacted morula Weak 2B m-/- Continued to display higher levels of Eed and H3K27me3 than the Eed mz-/- embryos, but lower levels than Eed fl/fl embryos (summarized in 2C and 2E).
Specimen 2B 16-cell mz-/-: embryonic day 3.0; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline. Double labeled: red - Eed; green - H3K27me3.
Structure Level Pattern Image Note
TS4: compacted morula Absent 2B mz-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory