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Gene Expression Data
Assay Details
Assay
Reference: J:331972 Hummel D, et al., Celsr1 suppresses Wnt5a-mediated chemoattraction to prevent incorrect rostral migration of facial branchiomotor neurons. Development. 2022 Nov 15;149(22):dev200553
Assay type: RNA in situ
MGI Accession ID: MGI:7495495
Gene symbol: Tbx20
Gene name: T-box 20
Probe: Tbx20 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2E: embryonic day 12.5 (more )
Note: Embryos obtained from double heterozygote crosses.
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 2E Expressed in areas spanning r4 to r6.
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted 2E Expressed in trigeminal motor neurons (asterisk).
Specimen 2F: embryonic day 12.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Embryos obtained from double heterozygote crosses.
Structure Level Pattern Image Note
TS20: hindbrain lateral wall Present Regionally restricted 2F All neurons migrated caudally into r5 and r6.
Specimen 2G: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1tm1.1Fati (more )
Note: Embryos obtained from double heterozygote crosses.
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 2G A large number of neurons migrated rostrally into r3 and r2 (arrowheads).
Specimen 2H: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1tm1.1Fati, Wnt5atm1Amc/Wnt5atm1Amc (more )
Note: Embryos obtained from double heterozygote crosses.
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 2H Rostral inappropriate migration was almost completely suppressed in double mutants.
Specimen 3D: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 3D Expressed in rhombomere 4 (r4) to r6.
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted 3D Expressed in rhombomere 2 (asterisks).
Specimen 3E: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1+ (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 3E Expressed in rhombomere 4 (r4) to r6, similar as in the wild-type (quantified in Fig 3J).
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted 3E Expressed in rhombomere 2 (asterisks).
Specimen 3F: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1tm1.1Fati (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 3F Dramatic increase in rostral migration. Neurons migrated rostrally into r2 and beyond (quantified in Fig 3J, S7C).
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted 3F Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.
Specimen 3G: embryonic day 12.5; Egr2tm2(cre)Pch/Egr2+, Gt(ROSA)26Sortm1(CAG-Wnt5a,-AcGFP)Skde/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 3G A significant number of positive neurons were located in r3 (quantified in Fig 3J, S7A).
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted 3G Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.
Specimen 3H: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1+, Egr2tm2(cre)Pch/Egr2+, Gt(ROSA)26Sortm1(CAG-Wnt5a,-AcGFP)Skde/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 3H A large number of positive neurons were located in r3 (quantified in Fig 3J, S7B), with neurons spilling over into r2.
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted 3H Expressed in rhombomere 2 (asterisks).
Specimen 3I: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1tm1.1Fati, Egr2tm2(cre)Pch/Egr2+, Gt(ROSA)26Sortm1(CAG-Wnt5a,-AcGFP)Skde/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted 3I Neurons migrated extensively into r3. Although more numerous, appeared to remain largely within r3 (quantified in Fig 3J, S7D). There was a marked reduction in the size of the caudal migratory stream.
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted 3I Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.
Specimen S3A: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted S3A Expressed in rhombomere 4 (r4) to r6.
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted S3A Expressed in rhombomere 2 (asterisks).
Specimen S3B: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1+ (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted S3B Expressed in rhombomere 2 (asterisks), similar as in the wild-type.
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted S3B Expressed mainly in rhombomere 4 (r4) to r6. Slightly migration into r3 was seen.
Specimen S3C: embryonic day 12.5; Celsr1tm1.1Fati/Celsr1tm1.1Fati (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted S3C Neurons migrated rostrally into r3 and r2.
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted S3C Expressed in rhombomere 2 (asterisks), similar as in the wild-type.
Specimen S3D: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted S3D Expressed in rhombomere 2 (asterisks).
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted S3D Expressed in rhombomere 4 (r4) to r6.
Specimen S3E: embryonic day 12.5; Wnt5atm1Amc/Wnt5a+ (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted S3E Expressed in rhombomere 2 (asterisks), similar as in the wild-type.
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted S3E Expressed in rhombomere 4 (r4) to r6, similar as in the wild-type.
Specimen S3F: embryonic day 12.5; Wnt5atm1Amc/Wnt5atm1Amc (more )
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted S3F Expressed in rhombomere 2 (asterisks), similar as in the wild-type.
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted S3F Expressed in rhombomere 4 (r4) to r6, similar as in the wild-type.
Specimen S6A: embryonic day 12.5 (more )
Note: wild-type control
Structure Cell Type Level Pattern Image Note
TS20: hindbrain lateral wall trigeminal motor neuron Present Regionally restricted S6A Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.
TS20: hindbrain lateral wall branchiomotor neuron Present Regionally restricted S6A Expressed in rhombomere 4 (r4) to r6.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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08/05/2025
MGI 6.24 		The Jackson Laboratory