Gene Expression Data
Assay Details
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Assay
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Reference:
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J:330741
Orchard P, et al., Genome-wide chromatin accessibility and transcriptome profiling show minimal epigenome changes and coordinated transcriptional dysregulation of hedgehog signaling in Danforth's short tail mice. Hum Mol Genet. 2019 Mar 1;28(5):736-750
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7491735
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 2%: 25% glutaraldehyde in PBS with a post fix with 4% paraformaldehyde.
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Results
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Specimen
4H:
embryonic day 10.5; Gli1tm2Alj/Gli1 +
(more  )
Specimen
4H:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1 * Danforth's posterior duplication stock
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Mutant Allele(s):
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Gli1tm2Alj/Gli1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail |
Present |
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4H
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Specimen
4I:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1 * Danforth's posterior duplication stock * SD
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Mutant Allele(s):
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Etn2Sd/Etn2+
Gli1tm2Alj/Gli1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail |
Weak |
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4I
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Analysis Tools
