Probe:	Chd7 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	1B	Expression in most embryonic tissues.
TS20: nervous system	Strong	Regionally restricted	1B	High, localized expression specifically in nervous system.
TS20: brain	Present	Regionally restricted	1B	Expression throughout neuroepithelium of developing central nervous system. Expression in ventricular region.
TS20: spinal cord	Present	Regionally restricted	1B	Expression throughout neuroepithelium of developing central nervous system. Expression in ventricular region.
TS20: cerebellum primordium	Present		1B
TS20: neocortex	Present		1B
TS20: diencephalon	Present		1B
TS20: pituitary gland	Present		1B
TS20: tongue	Present	Diffuse	1B
TS20: paraxial mesenchyme	Present	Regionally restricted	1B	Expression in intersomitic mesoderm.
TS20: dorsal root ganglion	Present		1B
TS20: gut	Present		1B
TS20: lung	Present		1B
TS20: tail	Present		1B

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	1Ba	Expression throughout neuroepithelium. Expression in floor plate.
TS20: lower rhombic lip	Present		1Ba
TS20: upper rhombic lip	Present		1Ba

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	1Bb	Higher expression on ventricular side.
TS20: cerebral cortex subventricular zone	Present	Regionally restricted	1Bb	Expression in both ventricular and subventricular zones.
TS20: cerebral cortex ventricular layer	Present	Regionally restricted	1Bb	Expression in both ventricular and subventricular zones.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	S1E	Expression throughout length of neural tube. Expression in both cranial and caudal poles. Distinct enrichment in ventricular zone.
TS20: dorsal root ganglion	Present		S1E

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	S1F	Expression throughout length of neural tube. Expression in both cranial and caudal poles. Distinct
TS20: paraxial mesenchyme	Present	Regionally restricted	S1F	Expression in intersomitic regions.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	S1G	Expression enriched in ventricular zone. Ventral to dorsal gradient of expression within spinal cord.
TS20: dorsal root ganglion	Present		S1G
TS20: eye	Present		S1G

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	S1H	Expression throughout length of neural tube. Ventral to dorsal gradient of expression within spinal cord.
TS20: dorsal root ganglion	Present		S1H

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present		2F
TS20: vestibulo-cochlear ganglion	Present		2F

Structure	Level	Pattern	Image	Note
TS20: metanephros	Strong	Regionally restricted	2G	High expression in mesenchyme and metanephric tubule epithelium.
TS20: cap mesenchyme	Present		2G

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present		2H

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present		2I
TS20: pigmented retinal epithelium	Present		2I

Structure	Level	Pattern	Image	Note
TS20: tooth bud	Absent			No expression in incisor primordium at E12.5.

Structure	Level	Pattern	Image	Note
TS22: incisor	Present		2J	Expression in incisor primordium at E14.5.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Widespread	3B	Distinct expression patterns throughout embryo.
TS22: nervous system	Strong	Regionally restricted	3B	Notably higher levels in developing nervous system.
TS22: diencephalon	Present		3B
TS22: midbrain	Present		3B
TS22: spinal cord	Present	Widespread	3B
TS22: tongue	Present		3B
TS22: heart	Present		3B
TS22: liver	Present		3B
TS22: lung	Present		3B
TS22: metanephros	Present		3B
TS22: thymus primordium	Present		3B

Structure	Level	Pattern	Image	Note
TS22: cerebellum ventricular layer	Present		3Ba
TS22: rhombic lip	Present		3Ba

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Strong	Regionally restricted	3Bb	Most prominent expression in ventricular region of neocortex.
TS22: neocortex	Strong	Regionally restricted	3Bb	Most prominent expression in ventricular region of neocortex.

Structure	Level	Pattern	Image	Note
TS22: lens	Present		3Bc
TS22: neural retina	Present		3Bc
TS22: retina	Strong		3Bc	Particularly strong expresion in retina.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		3Bd

Structure	Level	Pattern	Image	Note
TS22: cap mesenchyme	Present		3Be	Expression in condensing mesenchyme of kidney.

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Widespread	4B
TS27: cerebellum	Strong		4B
TS27: pons	Strong		4B

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Widespread	4B'
TS27: cerebellum	Strong		4B'
TS27: pons	Strong		4B'

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		4Ba

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present		4Bb

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5B
TS28: cerebellum	Present		5B
TS28: hippocampus	Present		5B
TS28: neocortex	Present		5B

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5B'
TS28: cerebellum	Present		5B'
TS28: hippocampus	Present		5B'
TS28: main olfactory bulb	Present		5B'
TS28: neocortex	Present		5B'
TS28: rostral migratory stream	Present		5B'

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		5Ba

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		5Bb
TS28: hippocampus CA1	Present		5Bb
TS28: hippocampus CA2	Present		5Bb
TS28: hippocampus CA3	Present		5Bb

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5Bc
TS28: cortical layer III	Present		5Bc

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		6B
TS28: hippocampus	Weak		6B
TS28: rostral migratory stream	Weak		6B

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		6B'
TS28: main olfactory bulb	Weak		6B'

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		6Ba

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		6Bb
TS28: hippocampus CA1	Present		6Bb
TS28: hippocampus CA2	Present		6Bb
TS28: hippocampus CA3	Present		6Bb
TS28: hippocampus	Weak	Diffuse	6Bb	Expression in hippocampus lower and more diffuse compared to that of Chd8.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Trace		6Bc	Very low expression in neocortex compared to that of Chd8 and Chd2.