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Gene Expression Data
Assay Details
Assay
Reference: J:192269 Mukhopadhyay A, et al., Negative regulation of Shh levels by Kras and Fgfr2 during hair follicle development. Dev Biol. 2013 Jan 15;373(2):373-82
Assay type: Western blot
MGI Accession ID: MGI:7484067
Gene symbol: Mapk1
Gene name: mitogen-activated protein kinase 1
Antibody: anti-p-ERK1/2 (Thr202/Tyr204)
Detection system: Secondary antibody coupled to other - see notes
Assay notes: Secondary antibody was labeled with IRDye 680CW. The antibody used recognizes the phosphorylated form of the protein.
Results
Image: 4A pERK ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 42 kDa (a) Genetic BackgroundMutant Allele(s)Sex Note
wildtype 1 P7 TS28: skin Present Not Specified Not Specified dorsal skin
wildtype 2 P7 TS28: skin Present Not Specified Not Specified dorsal skin
wildtype 3 P7 TS28: skin Present Not Specified Not Specified dorsal skin
wildtype 4 P7 TS28: skin Present Not Specified Not Specified dorsal skin
wildtype 5 P7 TS28: skin Present Not Specified Not Specified dorsal skin
KrasG12D 1 P7 TS28: skin Present involves: 129S4/SvJae * C57BL/6 * CBA Krastm4Tyj/Kras+, Tg(Msx2-cre)5Rem/0Not Specified Conditional mutant. dorsal skin
KrasG12D 2 P7 TS28: skin Present involves: 129S4/SvJae * C57BL/6 * CBA Krastm4Tyj/Kras+, Tg(Msx2-cre)5Rem/0Not Specified Conditional mutant. dorsal skin
KrasG12D 3 P7 TS28: skin Present involves: 129S4/SvJae * C57BL/6 * CBA Krastm4Tyj/Kras+, Tg(Msx2-cre)5Rem/0Not Specified Conditional mutant. dorsal skin
KrasG12D 4 P7 TS28: skin Present involves: 129S4/SvJae * C57BL/6 * CBA Krastm4Tyj/Kras+, Tg(Msx2-cre)5Rem/0Not Specified Conditional mutant. dorsal skin
KrasG12D 5 P7 TS28: skin Present involves: 129S4/SvJae * C57BL/6 * CBA Krastm4Tyj/Kras+, Tg(Msx2-cre)5Rem/0Not Specified Conditional mutant. dorsal skin
Notes:
(a) No significant change between the wild-type and mutant after normalized with loading control (summarized in Fig 4B).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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