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Gene Expression Data
Assay Details
Assay
Reference: J:333869 Nourse JL, et al., Piezo1 regulates cholesterol biosynthesis to influence neural stem cell fate during brain development. J Gen Physiol. 2022 Oct 3;154(10)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7481980
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-B3 tubulin (2G10): sc-80005
Detection system: Secondary antibody
Results
Specimen 2A - WT forebrain: embryonic day 10.5 (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: forebrain neuron of the forebrain Present 2A WT Forebrain merge
Specimen 2A - WT midbrain: embryonic day 10.5 (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: midbrain central nervous system neuron Present 2A WT Midbrain merge
Specimen 2A/S1 - WT hindbrain: embryonic day 10.5 (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: hindbrain central nervous system neuron Present S1_WT merge , 2A WT Hindbrain merge
Specimen 2A - KO midbrain: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: midbrain neuron of the forebrain Present 2A KO Midbrain merge
Specimen S1 - KO hindbrain: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: hindbrain central nervous system neuron Present S1_KO merge
Specimen 2B - WT: embryonic day 10.5 (more )
Note: Triple labeled: yellow - Tubb3; cyan - Nes (assay MGI:7481977); magenta - Sox2 (assay MGI:7481991).
Structure Cell Type Level Pattern Image Note
TS17: midbrain central nervous system neuron Present 2B WT TUJ1 , 2B WT merge
Specimen 2B - KO: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: yellow - Tubb3; cyan - Nes (assay MGI:7481977); magenta - Sox2 (assay MGI:7481991).
Structure Cell Type Level Pattern Image Note
TS17: midbrain central nervous system neuron Present 2B KO TUJ1 , 2B KO merge Expression is noticeably diminished in the mutants; there is less staining and a reduction in axonal fibers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory