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Gene Expression Data
Assay Details
Assay
Reference: J:333869 Nourse JL, et al., Piezo1 regulates cholesterol biosynthesis to influence neural stem cell fate during brain development. J Gen Physiol. 2022 Oct 3;154(10)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7481977
Gene symbol: Nes
Gene name: nestin
Antibody: anti-nestin (ab134017)
Detection system: Secondary antibody
Results
Specimen 2B - WT: embryonic day 10.5 (more )
Note: Triple labeled: cyan - Nes; yellow - Tubb3 (assay MGI:7481980); magenta - Sox2 (assay MGI:7481991).
Structure Cell Type Level Pattern Image Note
TS17: midbrain neural stem cell Present 2B WT merge , 2B WT NESTIN Expressing cells are organized along an apical-basal axis.
Specimen 2B - KO: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: cyan - Nes; yellow - Tubb3 (assay MGI:7481980); magenta - Sox2 (assay MGI:7481991).
Structure Cell Type Level Pattern Image Note
TS17: midbrain neural stem cell Present 2B KO NESTIN , 2B KO merge In wild type expressing cells are organized along an apical-basal axis; this organization is disrupted in the mutants.
Specimen S2 - WT: embryonic day 10.5 (more )
Structure Cell Type Level Pattern Image Note
TS17: hindbrain neural stem cell Present S2_WT red inset , S2_WT white inset , S2_WT NESTIN Expressing cells are organized along an apical-basal axis.
Specimen S2 - KO: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Structure Cell Type Level Pattern Image Note
TS17: hindbrain neural stem cell Present S2_KO red inset , S2_KO NESTIN , S2_KO white inset In wild type expressing cells are organized along an apical-basal axis; this organization is disrupted in the mutants.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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