About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:333869 Nourse JL, et al., Piezo1 regulates cholesterol biosynthesis to influence neural stem cell fate during brain development. J Gen Physiol. 2022 Oct 3;154(10)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7481980
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-B3 tubulin (2G10): sc-80005
Detection system: Secondary antibody
Results
Specimen 2A - WT forebrain: embryonic day 10.5 (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: forebrain neuron of the forebrain Present 2A WT Forebrain merge
Specimen 2A - WT midbrain: embryonic day 10.5 (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: midbrain central nervous system neuron Present 2A WT Midbrain merge
Specimen 2A/S1 - WT hindbrain: embryonic day 10.5 (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: hindbrain central nervous system neuron Present S1_WT merge , 2A WT Hindbrain merge
Specimen 2A - KO midbrain: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: midbrain neuron of the forebrain Present 2A KO Midbrain merge
Specimen S1 - KO hindbrain: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: magenta - Tubb3; yellow - Piezo1 (assay MGI:7481975); cyan - Hoechst.
Structure Cell Type Level Pattern Image Note
TS17: hindbrain central nervous system neuron Present S1_KO merge
Specimen 2B - WT: embryonic day 10.5 (more )
Note: Triple labeled: yellow - Tubb3; cyan - Nes (assay MGI:7481977); magenta - Sox2 (assay MGI:7481991).
Structure Cell Type Level Pattern Image Note
TS17: midbrain central nervous system neuron Present 2B WT TUJ1 , 2B WT merge
Specimen 2B - KO: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: yellow - Tubb3; cyan - Nes (assay MGI:7481977); magenta - Sox2 (assay MGI:7481991).
Structure Cell Type Level Pattern Image Note
TS17: midbrain central nervous system neuron Present 2B KO TUJ1 , 2B KO merge Expression is noticeably diminished in the mutants; there is less staining and a reduction in axonal fibers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/28/2024
MGI 6.13 		The Jackson Laboratory