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Gene Expression Data
Assay Details
Assay
Reference: J:207609 Hilliard SA, et al., Mdm2 is required for maintenance of the nephrogenic niche. Dev Biol. 2014 Mar 1;387(1):1-14
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7481344
Gene symbol: Pax2
Gene name: paired box 2
Antibody: anti Pax2 Z-RX2 (71-6000)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 8A/D: embryonic day 14.5 (more )
Note: Double labeled: red - Pax2; green - Lhx1 (assay MGI:7481345).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 8D , 8A
TS22: early nephron Present 8D , 8A
Specimen 8B/E: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Pax2; green - Lhx1 (assay MGI:7481345).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 8E , 8B Reduced expression compared with the control.
TS22: early nephron Present 8E , 8B There were fewer (mild) positive nephron intermediates.
Specimen 8C/F: embryonic day 14.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Pax2; green - Lhx1 (assay MGI:7481345).
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 8C , 8F Reduced expression compared with the control.
TS22: early nephron Present 8F , 8C There were fewer (severe) positive nephron intermediates.
Specimen 11J: postnatal day 0 (more )
Note: Double labeled: red - Pax2; green - Lhx1 (assay MGI:7481345).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11J
TS27: early nephron Present 11J
Specimen 11K: postnatal day 0; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Pax2; green - Lhx1 (assay MGI:7481345).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11K Reduced expression compared with the control.
TS27: early nephron Present 11K Reduced expression compared with the control.
Specimen 11L: postnatal day 0; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0, Trp53tm1Tyj/Trp53tm1Brn (more )
Note: Conditional mutant. Double labeled: red - Pax2; green - Lhx1 (assay MGI:7481345).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present 11L The rescued kidneys showed well developed nephrogenic zone.
TS27: early nephron Present 11L The rescued kidneys showed well developed nephrogenic zone.
Specimen S2E: embryonic day 12.5 (more )
Note: Double labeled: red - Pax2; green - pan cytokeratin.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present S2E
Specimen S2F: embryonic day 12.5; Mdm2tm1.2Mep/Mdm2tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Pax2; green - pan cytokeratin.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present S2F Smaller cap mesenchyme compared with the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory