Antibody:	anti-osteocalcin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The specificity of the antibody used was not detailed; both family members have been annotated.

Structure	Level	Pattern	Image	Note
TS28: mandible condylar process	Present		2E lower WT , 2E upper WT

Structure	Level	Pattern	Image	Note
TS28: mandible condylar process	Present		2E upper cKO , 2E lower cKO	Similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS28: head of femur	Present		3E upper WT , 3E lower WT

Structure	Level	Pattern	Image	Note
TS28: head of femur	Present		3E upper cKO , 3E lower cKO	Higher expression in the cortical bone (quantified in Fig 3G).

Structure	Level	Pattern	Image	Note
TS28: femur	Present		4F lower WT left , 4F upper WT , 4F lower WT right

Structure	Level	Pattern	Image	Note
TS28: femur	Present		4F lower cKO right , 4F upper cKO , 4F lower cKO left	Higher expression in the cortical bone, compared with the wild-type.