Gene Expression Data
Assay Details
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Assay
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Reference:
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J:89882
Hansen TV, et al., Dwarfism and impaired gut development in insulin-like growth factor II mRNA-binding protein 1-deficient mice. Mol Cell Biol. 2004 May;24(10):4448-64
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7469601
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Antibody:
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anti-PCNA
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
7Ba:
embryonic day 17.5
(more  )
Specimen
7Ba:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: liver |
Present |
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7Ba
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Specimen
7Bb:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Igf2bp1Gt(OST33739)Lex/Igf2bp1Gt(OST33739)Lex
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: liver |
Present |
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7Bb
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In this mutant the number of positive cells was reduced from 33% to 23%.
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Specimen
7Be:
embryonic day 17.5
(more )
Specimen
7Be:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: small intestine |
Present |
Regionally restricted |
7Be
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Positive cells were mainly located at the root of the villi.
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Specimen
7Bf:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Igf2bp1Gt(OST33739)Lex/Igf2bp1Gt(OST33739)Lex
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: small intestine |
Present |
Regionally restricted |
7Bf
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In this mutant positive cells were abundant all over the imperfectly developed villi.
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Specimen
not shown WT:
embryonic day 17.5
(more )
Specimen
not shown WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown -/-:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Igf2bp1Gt(OST33739)Lex/Igf2bp1Gt(OST33739)Lex
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metanephros |
Present |
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In this mutant the number of positive cells was reduced from 27% to 16%.
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Analysis Tools