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Gene Expression Data
Assay Details
Assay
Reference: J:208435 Yi SH, et al., Foxa2 acts as a co-activator potentiating expression of the Nurr1-induced DA phenotype via epigenetic regulation. Development. 2014 Feb;141(4):761-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7468764
Gene symbol: Nr4a2
Gene name: nuclear receptor subfamily 4, group A, member 2
Antibody: Anti-Nurr1/Nur77 (E20)
Detection system: Secondary antibody coupled to Cy5
Results
Specimen 1B/C: embryonic day 9.0 (more )
Note: Double labeled: red - Nr4a2; green - Foxa2 (assay MGI:7468756).
Structure Level Pattern Image Note
TS14: future midbrain Absent 1C , 1B
Specimen 1E/F: embryonic day 12.0 (more )
Note: Double labeled: red - Nr4a2; green - Foxa2 (assay MGI:7468756).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present Regionally restricted 1F , 1E Expressed in the intermediate and mantle zone of the ventral region, largely colocalized with Foxa2.
TS20: midbrain ventricular layer Absent 1E , 1F
Specimen 1H/I: embryonic day 15.0 (more )
Note: Double labeled: red - Nr4a2; green - Foxa2 (assay MGI:7468756).
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 1I , 1H Expressed in the ventral region.
Specimen 1M: embryonic day 12.0 (more )
Note: Triple labeled: red - Nr4a2; blue - Th (assay MGI:7468767); green - Foxa2 (assay MGI:7468756).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 1M
TS20: midbrain ventricular layer Absent 1M
Specimen 5A: embryonic day 12.0 (more )
Note: Double labeled: red - Nr4a2; green - Foxa2 (assay MGI:7468756).
Structure Cell Type Level Pattern Image Note
TS20: midbrain mantle layer midbrain dopaminergic neuron Present 5A Nurr1 , 5A merge A granular distribution within the nucleus was shown, coexpressed with Foxa2 (taken from Th+ cells in Fig 1M).
Specimen 6A/a: embryonic day 12.5 (more )
Note: Double labeled: red - Nr4a2; green - Rcor1 (assay MGI:7468773).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 6A left Coexpressed with Rcor1.
Specimen S1G: embryonic day 12.5 (more )
Note: Double labeled: red - Nr4a2; green - Foxa2 (assay MGI:7468756).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present S1G left inset , S1G right Coexpressed with Foxa2 in the ventral region.
Specimen S3 Nurr1/Pitx3: embryonic day 12.0 (more )
Note: Double labeled: red - Nr4a2; green - Pitx3 (assay MGI:7468779).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present S3_left Coexpressed with Pitx3 in the nuclei.
Specimen S3 Nurr1/HuC/D: embryonic day 12.0 (more )
Note: Double labeled: red - Nr4a2; green - Elavl3 (assay MGI:7468783) and Elavl4 (assay MGI:7468837).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present S3_middle Expressed in the nuclei.
Specimen S3 Nurr1/NeuN: embryonic day 12.0 (more )
Note: Double labeled: red - Nr4a2; green - Rbfox3 (assay MGI:7468786).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present S3_right Coexpressed with Rbfox3 in the nuclei.
Specimen not shown: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: midbrain midbrain dopaminergic neuron Present Exclusively colocalized in Th+ dopaminergic neurons.
TS28: hippocampus hippocampal CA1-3 neuron Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory